Knowledge of stenosis in coronary arteries requires an understanding of the cellular and molecular processes that occur throughout the leukocyte rolling process. In this study, the roles of miR-125a-5p and miR-495-3p were investigated on the adhesion of endothelial cells (ECs) isolated from the human aorta. Human primary endothelial cells were obtained from the aorta of people who had died of brain death. Whole blood was used to isolate the monocytes. The miR-125 and miR-495 were predicted and transfected into ECs using Poly Ethylene Imine (PEI). The expression levels of adhesion molecules and monocyte recruitment were identified by the RT-qPCR technique and Leukocyte-Endothelial Adhesion Assay kit, respectively. The ICAM-1, ICAM-2 and VCAM-1 expression levels decreased significantly in the miR-495/PEI-transfected ECs (P < 0.05) while in the miR-125/PEI-transfected ECs only the ICAM-2 and ITGB-2 expression levels decreased significantly (P < 0.05) as compared to the miR-synthetic/PEI-transfected ECs. Furthermore, the monocyte adhesion was decreased in the miR-125 and miR-mix/PEI-transfected ECs as compared to the miR-synthetic/PEI-transfected ECs (P = 0.01 and P = 0.04, respectively). According to the findings, the efficient relations between miR-125 and adhesion molecules may be responsible for the inhibition of monocyte rolling.

中文翻译：

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从人主动脉分离的单核细胞和内皮细胞的粘附被 miRNA-PEI 颗粒抑制

冠状动脉狭窄的知识需要了解在整个白细胞滚动过程中发生的细胞和分子过程。在这项研究中，研究了 miR-125a-5p 和 miR-495-3p 对从人主动脉中分离出的内皮细胞 (EC) 粘附的作用。人类原代内皮细胞是从死于脑死亡的人的主动脉中获得的。全血用于分离单核细胞。使用聚乙烯亚胺 (PEI) 预测 miR-125 和 miR-495 并将其转染到 ECs 中。粘附分子和单核细胞募集的表达水平分别通过 RT-qPCR 技术和白细胞-内皮粘附检测试剂盒进行鉴定。miR-495/PEI 转染的内皮细胞中 ICAM-1、ICAM-2 和 VCAM-1 的表达水平显着降低（P < 0. 05) 而在 miR-125/PEI 转染的 ECs 中，与 miR-125/PEI 转染的 ECs 相比，只有 ICAM-2 和 ITGB-2 的表达水平显着降低（P < 0.05）。此外，与 miR-125 和 miR-mix/PEI 转染的 ECs 相比，单核细胞粘附降低（分别为 P = 0.01 和 P = 0.04）。根据研究结果，miR-125与粘附分子之间的有效关系可能是抑制单核细胞滚动的原因。分别）。根据研究结果，miR-125与粘附分子之间的有效关系可能是抑制单核细胞滚动的原因。分别）。根据研究结果，miR-125与粘附分子之间的有效关系可能是抑制单核细胞滚动的原因。