ABSTRACT

In the present study, a set of ten Expressed Sequence Tag-Simple Sequence Repeats (EST-SSR) markers have been developed to contribute to the genetic studies of Pinus thunbergii. The number of alleles in the developed EST-SSR markers ranged from one to six. Observed heterozygosity was 0.000 to 0.707; the expected heterozygosity ranged from 0.000 to 0.720. Polymorphism information content (PIC) was ranged from 0.000 to 0.669. The mean of the probability of identity between two unrelated individuals (PI) and two siblings (PI-Sib) were 0.540 and 0.719, respectively. The EST-SSR markers developed in the present study, in combining with the previously developed markers, can help in understanding the genetic diversity and genetic structure of P. thunbergii populations.

摘要

在本研究中，已经开发了一组十个表达序列标签-简单序列重复 (EST-SSR) 标记，以促进松柏的遗传研究。开发的 EST-SSR 标记中的等位基因数量从 1 到 6。观察到的杂合度为 0.000 至 0.707；预期杂合度在 0.000 到 0.720 之间。多态信息含量 ( PIC ) 的范围从 0.000 到 0.669。两个无关个体 ( PI ) 和两个兄弟姐妹 ( PI-Sib )之间身份概率的均值) 分别为 0.540 和 0.719。本研究开发的 EST-SSR 标记与先前开发的标记相结合，有助于了解P. thunbergii种群的遗传多样性和遗传结构。