Abstract

Bilayer wound dressing systems consisting of various biopolymers have been preferred in biomedical applications due to its enhanced advantages in comparison with conventional systems. The aim of this research was to develop a novel bilayer wound dressing based on gelatin (G) and gellan gum (GG). The bilayer was composed of an upper layer impregnated with antibiotic drug sodium ampicillin and a drug-free lower sponge layer. The bilayer hydrogels were characterized by FTIR, TGA, DSC and SEM analyses including swelling behaviors and hydrolytic degradation tests. To achieve faster healing of the wound by prevention of the bacterial infection, the bilayer hydrogels were developed as antibiotic-releasing vehicles. Herein, release study of sodium ampicillin was performed in PBS to simulate the physiological micro-environment. Additionally, cyto-compatibility tests of L929 fibroblast cells showed the high proliferation and survival through drug-loaded GG and G hydrogels (GG-G-D) after 24h, 48h and 72h incubation. This novel GG-G bilayer hydrogel could be a good candidate as wound dressing.

摘要

由各种生物聚合物组成的双层伤口敷料系统由于与传统系统相比具有增强的优势，因此在生物医学应用中已成为首选。本研究的目的是开发一种基于明胶 (G) 和结冷胶 (GG) 的新型双层伤口敷料。双层由浸渍抗生素药物氨苄青霉素钠的上层和不含药物的下海绵层组成。通过 FTIR、TGA、DSC 和 SEM 分析对双层水凝胶进行了表征，包括溶胀行为和水解降解测试。为了通过预防细菌感染实现更快的伤口愈合，双层水凝胶被开发为抗生素释放载体。在此，在 PBS 中进行了氨苄青霉素钠的释放研究，以模拟生理微环境。此外，L929 成纤维细胞的细胞相容性试验表明，在 24 小时、48 小时和 72 小时孵育后，通过载药 GG 和 G 水凝胶 (GG-GD) 的高增殖和存活率。这种新型 GG-G 双层水凝胶可以作为伤口敷料的良好候选者。