Aeromonas veronii (A. veronii, AV) strains are emerging zoonotic and aquatic pathogens, yet we know very little about their genomics. This study aims to utilize comparative genomics to investigate the intraspecific genetic diversity, differences in virulence factors and evolutionary mechanisms of A. veronii strains from diverse sources and to fundamentally demonstrate their pathogenic mechanisms. We conducted comparative genomics analysis of 39 A. veronii strains from different sources and found that 1993 core genes are shared by these strains and that these shared core genes may be necessary to maintain the basic characteristics of A. veronii. Additionally, phylogenetic relationship analysis based on these shared genes revealed that a distant relationship between the AMC34 strain and the other 38 strains but that, the genetic relationship among the 38 strains is relatively close, indicating that AMC34 may not belong to A. veronii. Furthermore, analysis of shared core genes and average nucleotide identity (ANI) values showed no obvious correlation with the location of A. veronii isolation and genetic relationship. Our research indicates the evolutionary mechanism of A. veronii from different sources and provides new insights for a deeper understanding of its pathogenic mechanism.

维罗单胞菌( A. veronii, AV ) 菌株是新兴的人畜共患和水生病原体，但我们对它们的基因组学知之甚少。本研究旨在利用比较基因组学研究不同来源维氏曲霉菌的种内遗传多样性、毒力因子差异和进化机制，并从根本上证明其致病机制。我们对来自不同来源的 39株维氏曲霉进行了比较基因组学分析，发现这些菌株共有 1993 个核心基因，而这些共有的核心基因可能是维持维氏曲霉基本特征所必需的。此外，基于这些共享基因的系统发育关系分析表明，AMC34株与其他38株的亲缘关系较远，但38株之间的亲缘关系相对较近，表明AMC34可能不属于A. veronii。此外，对共享核心基因和平均核苷酸同一性 (ANI) 值的分析表明，与A. veronii分离位置和遗传关系没有明显的相关性。我们的研究从不同来源揭示了A. veronii的进化机制，并为更深入地了解其致病机制提供了新的见解。