Listeria monocytogenes crossing the blood-brain barrier in the form of “Trojan Horse” is of great significance for the establishment of bacterial encephalitis and meningitis. Induction of cell migration and crossing the blood-brain barrier is very important to understand the Listeria pathogenesis. The Rho GTPases family is considered a key factor in regulating cell migration. This study was designed to investigate the expression of Rho GTPases and their effect on the behavior of cell migration and the stimulation of immune factors. Selective Rho GTPases were investigated by real-time PCR and Western blot. Among these, the expression of RhoA was significantly increased following the infection of Listeria monocytogenes in macrophages. Further, we found that RhoA improves the migration of macrophages and expression of IL-1β, IL-6, and TNF-α. The expression of IL-1β, IL-6 and TNF-α possibly facilitates the migration and adhesion of macrophages to cross the blood-brain barrier. This study provides preliminary ground to investigate the detailed mechanism of Listeria monocytogenes crossing the blood-brain barrier.

单核细胞增生李斯特菌以“特洛伊木马”的形式穿越血脑屏障，对于细菌性脑炎和脑膜炎的建立具有重要意义。诱导细胞迁移和穿越血脑屏障对于了解李斯特菌的发病机制非常重要。Rho GTPases 家族被认为是调节细胞迁移的关键因素。本研究旨在研究 Rho GTPases 的表达及其对细胞迁移行为和免疫因子刺激的影响。通过实时 PCR 和蛋白质印迹研究选择性 Rho GTP 酶。其中，单核细胞增生李斯特菌感染后RhoA的表达显着增加在巨噬细胞中。此外，我们发现 RhoA 可改善巨噬细胞的迁移和 IL-1β、IL-6 和 TNF-α 的表达。IL-1β、IL-6 和 TNF-α 的表达可能促进巨噬细胞迁移和粘附穿过血脑屏障。该研究为研究单核细胞增生李斯特菌穿越血脑屏障的详细机制提供了初步基础。