The immunomodulatory antibacterial activity and osteoimmunomodulatory properties of implantable biomaterials significantly influence bone regeneration. Various types of ultraviolet (UV) instrument are currently in use to greatly enhance the antibacterial activity and osteoconductive capability of titanium, it remains unclear how UV treatment modulates immune response. Compared to traditional UV treatment, the combination of low-dose ozone with UV irradiation is considered a new option to give benefits to surface modification and reduce the drawbacks of UV and ozone individually. Herein, the aim of this study was to elucidate the immune-modulatory properties of macrophages on UV/ozone-irradiated titanium that serve as defense against S. aureus and the crosstalk between immune cells and osteoblasts. Three different cell and bacteria co-culture systems were developed in order to investigate the race between host cells and bacteria to occupy the surface. In vitro immunological experiments indicated that UV/ozone irradiation significantly enhanced the phagocytic and bactericidal activity of macrophages against S. aureus. Further, in vitro and in vivo studies evidenced the favorable osteoimmune environment for osteogenic differentiation and bone formation. This research suggests vital therapeutic potential of UV/ozone irradiation for preventing the biomaterial-associated infections and achieving favorable bone formation simultaneously.

可植入生物材料的免疫调节抗菌活性和骨免疫调节特性显着影响骨再生。目前使用各种类型的紫外线 (UV) 仪器来大大增强钛的抗菌活性和骨传导能力，但尚不清楚紫外线处理如何调节免疫反应。与传统的紫外线处理相比，低剂量臭氧与紫外线照射的结合被认为是一种新的选择，可以为表面改性带来好处，并分别减少紫外线和臭氧的缺点。在此，本研究的目的是阐明巨噬细胞对紫外线/臭氧照射的钛的免疫调节特性，这些钛可防御金黄色葡萄球菌以及免疫细胞和成骨细胞之间的串扰。开发了三种不同的细胞和细菌共培养系统，以研究宿主细胞和细菌之间的竞争以占据表面。体外免疫学实验表明，紫外线/臭氧照射显着增强了巨噬细胞对金黄色葡萄球菌的吞噬和杀菌活性。此外，体外和体内研究证明了有利于成骨分化和骨形成的骨免疫环境。这项研究表明，紫外线/臭氧辐射在预防生物材料相关感染和同时实现有利的骨形成方面具有重要的治疗潜力。