Abstract

The purpose of this study was to evaluate the neuroprotective effect of leptin on a non-human primate model of cerebral ischemia. A total of 39 Guangxi macaques were used to establish the primate cerebral-ischemia model. HE staining was used to evaluated the pathological changes. Moreover, magnetic resonance imaging was used for the detection of embolic area. The measurements of behavior observation and cerebral infarction area were also performed. They all received autologous thrombus operation. Furthermore, western blot and RT-PCR were also used to detect the protein and mRNA expression levels of apoptosis-related factors. Our results showed that leptin could reduce the volume of cerebral infarction by about 35%. Behavioral defects can be significantly improved. In addition, mid-term and long-term behavioral deficiencies had been significantly improved by leptin. Moreover, leptin significantly decreased the expression levels of caspase-3 and Bax, and increased the expression levels of Bcl-2. In conclusion, leptin has neuroprotective effects on cerebral ischemia by effectively reducing the volume of cerebral infarction.

摘要

本研究的目的是评估瘦素对脑缺血的非人灵长类动物模型的神经保护作用。共使用39只广西猕猴建立灵长类脑缺血模型。HE染色用于评估病理变化。此外，磁共振成像用于检测栓塞区域。还进行了行为观察和脑梗死面积的测量。他们都接受了自体血栓手术。此外，western blot和RT-PCR也被用来检测细胞凋亡相关因子的蛋白和mRNA表达水平。我们的研究结果表明，瘦素可使脑梗塞体积减少约 35%。行为缺陷可以得到显着改善。此外，瘦素显着改善了中长期行为缺陷。此外，瘦素显着降低了caspase-3和Bax的表达水平，并增加了Bcl-2的表达水平。综上所述，瘦素通过有效缩小脑梗死体积，对脑缺血具有神经保护作用。