Brain Research ( IF 2.7 ) Pub Date : 2021-08-14 , DOI: 10.1016/j.brainres.2021.147621 Arturo Avendaño-Estrada 1 , Camilo Rios 2 , Iñigo Aguirre-Aranda 3 , Miguel Ángel Ávila-Rodríguez 4 , Joaquín Manjarrez-Marmolejo 5 , Javier Franco-Pérez 5 , Juan Morales 1 , Roberto Olayo 1 , Marisela Méndez-Armenta 3 , Araceli Díaz-Ruíz 3
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Objective
Brain metabolic processes are not fully characterized in the kainic acid (KA)-induced Status Epilepticus (KASE). Thus, we evaluated the usefulness of 18F-fluorodeoxyglucose positron emission tomography (FDG-PET) as an experimental strategy to evaluate in vivo, in a non-invasive way, the glucose consumption in several brain regions, in a semi-quantitative study to compare and to correlate with data from electroencephalography and histology studies. Methods.
Sixteen male Wistar rats underwent FDG-PET scans at basal state and after KA injection. FDG-PET images were normalized to an MRI-based atlas and segmented to locate regions. Standardized uptake values (SUV) were obtained at several time points. EEGs and cell viability by histological analysis, were also evaluated.
Results
FDG-PET data showed changes in regions such as: amygdala, hippocampus, accumbens, entorhinal cortex, motor cortex and hypothalamus. Remarkably, hippocampal hypermetabolism was found (mean SUV = 2.66 ± 0.057) 2 h after KA administration, while hypometabolism at 24 h (mean SUV = 1.83 ± 0.056) vs basal values (mean SUV = 2.19 ± 0.057). EEG showed increased spectral power values 2 h post-KA administration. Hippocampal viable-cell counting 24 h after KA was decreased, while Fluoro-Jade B-positive cells were increased, as compared to control rats, coinciding with the hypometabolism detected in the same region by semi-quantitative FDG-PET at 24 h after KASE.
Conclusions
PET is suitable to measure metabolic brain changes in the rat model of status epilepticus induced by KA (KASE) at the first 24 h, compared to that of EEG; PET data may also be sensitive to cell viability.
中文翻译：
红藻氨酸诱导成年大鼠全脑早期代谢活性的表征：18FDG-PET研究
客观的
海人酸 (KA) 诱导的癫痫持续状态 (KASE) 并未完全表征脑代谢过程。因此，我们评估了18 F-氟脱氧葡萄糖正电子发射断层扫描 (FDG-PET) 作为一种实验策略的有用性，以非侵入性方式在体内评估几个大脑区域的葡萄糖消耗，在半定量研究中比较并关联来自脑电图和组织学研究的数据。方法。
16 只雄性 Wistar 大鼠在基础状态和 KA 注射后接受 FDG-PET 扫描。FDG-PET 图像被归一化为基于 MRI 的图谱并分割以定位区域。在几个时间点获得标准化摄取值 (SUV)。还通过组织学分析评估了脑电图和细胞活力。
结果
FDG-PET 数据显示以下区域的变化：杏仁核、海马、伏隔核、内嗅皮层、运动皮层和下丘脑。值得注意的是，KA 给药后 2 小时发现海马高代谢（平均 SUV = 2.66 ± 0.057），而 24 小时时的低代谢（平均 SUV = 1.83 ± 0.056）与基础值（平均 SUV = 2.19 ± 0.057）相比。脑电图显示 KA 给药后 2 小时光谱功率值增加。与对照大鼠相比，KA 后 24 小时海马活细胞计数减少，而 Fluoro-Jade B 阳性细胞增加，与 KASE 后 24 小时半定量 FDG-PET 在同一区域检测到的低代谢相一致.
结论
PET 适用于测量 KA (KASE) 诱发的大鼠癫痫持续状态模型在最初 24 小时内与 EEG 相比的代谢脑变化；PET 数据也可能对细胞活力敏感。
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