The current work demonstrates the use of a hybrid technique where ultrasound was coupled with three phase partitioning (UA-TPP) to intensify the extraction of imperatorin from fruits of A. marmelos. The optimized parameters obtained were ultrasound time 30 min, ammonium sulphate mass fraction 30 % (w/v), biomass to solvent proportion 1:40 (w/v), biomass slurry to t-butanol proportion 1:1 (v/v), ultrasound power 120 W, and duty cycle 60% (6 sec on 4 sec off). The maximum extraction yield of imperatorin obtained was 5.44 mg/g using UA-TPP, which was much higher than the traditional techniques such as three-phase partitioning (TPP), Soxhlet, and batch extraction. The extraction time was also drastically reduced to 30 min in UA-TPP from 150 min compared to TPP. UA-TPP enhanced the extraction yield of imperatorin with a reduction in time and improved the ethnomedicinal properties such as total phenolic and flavonoid content, antioxidant activity and anti-inflammatory activity as compared to traditional extraction techniques.

目前的工作证明了使用混合技术，其中超声与三相分配 (UA-TPP) 相结合，以加强从A. marmelos果实中提取欧前胡素. 获得的优化参数是超声时间 30 分钟，硫酸铵质量分数 30% (w/v)，生物质与溶剂比例 1:40 (w/v)，生物质浆液与叔丁醇比例 1:1 (v/v) ，超声功率 120 W，占空比 60%（6 秒开启 4 秒关闭）。使用 UA-TPP 获得的欧前胡素的最大提取率为 5.44 mg/g，远高于三相分配 (TPP)、索氏提取和批量提取等传统技术。与 TPP 相比，UA-TPP 的提取时间也从 150 分钟大幅减少到 30 分钟。与传统提取技术相比，UA-TPP 提高了欧前胡素的提取率，同时缩短了提取时间，并改善了总酚类和黄酮类化合物含量、抗氧化活性和抗炎活性等民族药用特性。