Objective

Some studies have implied the damaging effect of sevoflurane (sevo) on cognitive function in Alzheimer's disease (AD). This research was conducted to explore the effect of microRNA (miR)-132/forkhead-box A1 (FOXA1) axis on cognitive ability of sevo-treated AD rats.

Methods

The condensed-matter Aβ_1–40-induced AD rats were injected with miR-132- or FOXA1-related plasmids, followed by inhalation with 3% sevo. Then, the cognitive functions of AD rats were assessed. miR-132 and FOXA1 levels in hippocampal tissues of AD rats, and their interaction were identified.

Results

miR-132 expression was reduced and FOXA1 mRNA and protein levels were elevated in AD rats. miR-132 targeted FOXA1. Sevo treatment impaired cognitive function in AD rats. Elevated miR-132 or inhibited FOXA1 attenuated sevo-mediated injury in AD rats. Overexpressed FOXA1 rescued the effect of elevated miR-132 in AD rats with sevo treatment.

Conclusion

Up-regulated miR-132 reduces the cognition-damaging effect of sevo on AD rats by inhibiting FOXA1.

中文翻译：

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上调的 microRNA-132 通过抑制 FOXA1 降低七氟醚对阿尔茨海默病大鼠的认知损害作用

客观的

一些研究暗示了七氟醚 (sevo) 对阿尔茨海默病 (AD) 认知功能的破坏作用。本研究旨在探讨microRNA (miR)-132/forkhead-box A1 (FOXA1) 轴对 SEVO 治疗 AD 大鼠认知能力的影响。

方法

给凝聚态 Aβ _{1-40诱导的}AD 大鼠注射 miR-132 或 FOXA1 相关质粒，然后用 3% sevo 吸入_。然后，评估AD大鼠的认知功能。AD 大鼠海马组织中 miR-132 和 FOXA1 的水平，并确定了它们的相互作用。

结果

AD 大鼠中 miR-132 表达降低，FOXA1 mRNA 和蛋白质水平升高。miR-132 靶向 FOXA1。Sevo 治疗损害了 AD 大鼠的认知功能。升高的 miR-132 或抑制 FOXA1 可减轻 AD 大鼠中 sevo 介导的损伤。过表达的 FOXA1 挽救了用 sevo 治疗的 AD 大鼠中升高的 miR-132 的作用。

结论

上调的 miR-132 通过抑制 FOXA1 降低 sevo 对 AD 大鼠的认知损害作用。