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Aerobic granulation of single culture protist
Process Biochemistry ( IF 4.4 ) Pub Date : 2021-08-13 , DOI: 10.1016/j.procbio.2021.08.014
Zhaohui An 1 , Xueyao Zhang 1 , Yi Zheng 2 , Zhi-Wu Wang 1
Affiliation  

Thraustochytrium striatum was selected as a model protist to test the possibility of protist granulation for its good potential of organic waste valorization. Initially, only a selective settling velocity of 9 m h−1 was applied in a sequential batch reactor (SBR) with minimal granulation progress after three months. After the famine condition was introduced into the SBR with a feast-to-famine duration ratio of 0.2 by extending the cycle time and lowering the initial substrate concentration while keeping the selective settling velocity unchanged, protist granules with spherical shape and smooth surface formed and were dominated in the reactor after another month. The average particle size of protist granules was 728 μm, 90 times greater than its planktonic counterpart. This study evidenced the culture-independent phenomenon of aerobic granulation. Also, the combination of a low feast-to-famine duration ratio with a selective settling velocity was found to be required for inducing successful protist granulation.



中文翻译:

单培养原生生物好氧造粒

Thraustochytrium striatum被选为模型原生生物,以测试原生生物造粒的可能性,因为它具有良好的有机废物价值化潜力。最初,只有 9 mh -1的选择性沉降速度将其应用于连续间歇反应器 (SBR) 中,三个月后造粒进展最小。在保持选择性沉降速度不变的情况下,将饥荒条件以0.2的饥荒持续时间比引入SBR后,通过延长循环时间和降低初始底物浓度,形成球形和光滑表面的原生生物颗粒并又过了一个月,在反应堆中占据主导地位。原生生物颗粒的平均粒径为 728 μm,是其浮游生物颗粒的 90 倍。该研究证明了需氧造粒的培养独立现象。此外,发现需要将低盛宴与饥荒持续时间比与选择性沉降速度相结合才能诱导成功的原生生物颗粒化。

更新日期:2021-08-19
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