Label-free electrochemical biosensors are advantageous, over conventional bioassay techniques, offering rapid analytical response time, demands ultra-low sample volume, easy integration with modern gadgets useful for on-site utilities. However, achieving amplified sensitivity, reliability, and specificity without compromising inherent redox behavior is highly challenging. Indeed, developments in hybridized graphene nanostructure created new avenues for the construction of label-free electrochemical biosensor platforms. In this review, we present a comprehensive overview of the scalable fabrication demonstrated for hybridized graphene nanoarchitecture as label-free biosensor platform. Distinctly focused on electrodeposition and electroless deposition techniques utilized for the fabrication of hybridized graphene immuno-, nucleic acid- and enzyme-sensor platforms were discussed. Surface functionalization strategies investigated for anchoring bio-recognition elements on hybridized graphene materials were also presented, after introducing the fundamental properties. Lastly, we provide the advantages and drawbacks, highlighting the electrodeposited flexible hybrid graphene materials for future biosensor applications in point-of-care diagnosis.

与传统生物测定技术相比，无标记电化学生物传感器具有优势，可提供快速的分析响应时间，需要超低样品量，易于与用于现场公用事业的现代小工具集成。然而，在不影响固有氧化还原行为的情况下实现更高的灵敏度、可靠性和特异性是非常具有挑战性的。事实上，杂化石墨烯纳米结构的发展为构建无标记电化学生物传感器平台创造了新的途径。在这篇综述中，我们全面概述了混合石墨烯纳米结构作为无标记生物传感器平台的可扩展制造。明确专注于用于制造杂交石墨烯免疫的电沉积和无电沉积技术，讨论了核酸和酶传感器平台。在介绍了基本特性之后，还介绍了为将生物识别元件锚定在杂化石墨烯材料上而研究的表面功能化策略。最后，我们提供了优点和缺点，重点介绍了电沉积柔性混合石墨烯材料，用于未来生物传感器在即时诊断中的应用。