With the increasing importance of riboflavin in food, biological and pharmaceutical fields, accurately detecting its content is of great significance for guiding nutritional-health, diagnosis/treatment of related diseases, pharmacological research of riboflavin and supervision of food/drug quality. In this review, the recent detection methods established for riboflavin in food, biological and pharmaceutical fields were comprehensively summarized, including mass spectrometry, fluorescent detection, electrochemical detection, immunoassay, etc. Detailed information involving ingenious sample pretreatment, new-born/advanced materials, new established methods/sensors/platforms, advantages/disadvantages of various detection methodologies and eminent results are compared and discussed. With the elucidation of detection mechanism customized for riboflavin structure/physicochemical-property and the development of new-born functional materials for sensor/platform/sample-pretreatment, excellent detection performances with time-saving (separation-detection of riboflavin in urine within 2 min), high sensitivity (LOD as low as 0.5 fg mL⁻¹), eminent precision and accuracy for riboflavin analysis have been achieved. Finally, the current trends and future perspectives of riboflavin analysis are presented.

随着核黄素在食品、生物和制药领域的重要性日益提高，准确检测其含量对于指导营养健康、相关疾病的诊断/治疗、核黄素的药理研究和食品/药品质量监管具有重要意义。本综述全面总结了近年来在食品、生物和制药领域建立的核黄素检测方法，包括质谱、荧光检测、电化学检测、免疫分析等。比较和讨论了新建立的方法/传感器/平台，各种检测方法的优点/缺点和突出的结果。^-1 )，核黄素分析的卓越精密度和准确度已经实现。最后，介绍了核黄素分析的当前趋势和未来前景。