Former study suggests alkaloids from herbs of Aconitum genus plants possess excellent bioactivities, which exert great value for related deeper chemical constituent investigation. Herein, chemical isolation was performed and four alkaloids were isolated from Fuzi, of which two were new ones, and the other two were reported NMR data for the first time. Their chemical structures were identified by NMR data, high resolution MS, UV and IR analysis. Additionally, the MS fragmentation patterns were explored, formerly, that of hetisane alkaloid was rarely reported, and fragmentation mechanism of the diagnostic ion was proposed. Based on these fragment pathway, metabolites and metabolic pathways of four compounds were investigated in rat liver microsomes using UPLC−Q/TOF−MS, and dehydrogenation product was firstly found from metabolites of hetisane alkaloid.

中文翻译：

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附子中两种新生物碱及其代谢物研究

前人研究表明乌头属植物药材中的生物碱具有优良的生物活性，对相关更深入的化学成分研究具有重要价值。在此，进行化学分离，从附子中分离出四种生物碱，其中两个是新的，另外两个是首次报道的核磁共振数据。它们的化学结构通过核磁共振数据、高分辨率质谱、紫外和红外分析进行鉴定。此外，还探索了MS碎裂模式，以前很少报道hetisane生物碱的碎裂模式，并提出了诊断离子的碎裂机制。基于这些片段途径，使用UPLC-Q/TOF-MS在大鼠肝微粒体中研究了四种化合物的代谢物和代谢途径，并且首先从hetisane生物碱的代谢物中发现了脱氢产物。