In order to obtain and explore selenide composed of selenium and polysaccharide, three parameters were selected to optimize the synthesis process of selenium-Euryale ferox Salisb. polysaccharide (Se-ESPS) by Box-Behnken design. Furthermore, ESPS-B1 separated from ESPS was selenitized to Se-ESPS-B1 by the optimal synthesis process, then the characterization, and antioxidant activity in vitro of Se-ESPS-B1 were explored. The result manifested that the Se content of Se-ESPS was (2.915 ± 0.03) mg/g according to the optimal synthesis process of Se-ESPS (reaction time at 5 h, reaction temperature at 81 ℃, weight ratio of Na2SeO3 to ESPS at 0.9 g/g). A series of detection results indicated that the characterizations of Se-ESPS-B1 were apparently distinguished from that of ESPS-B1. Moreover, the antioxidant experiments in vitro demonstrated that Se-ESPS-B1 could exert antioxidant activity by scavenging DPPH, ABTS,·OH, and increasing reduction ability. In conclusion, the synthesis process is an effective approach to harvest selenium-polysaccharide, and Se-ESPS-B1 may be utilized as a potential antioxidant or selenium nutritional supplement.

中文翻译：

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硒-Euryale ferox Salisb 的合成、表征和体外抗氧化活性。多糖

为了获得和探索由硒和多糖组成的硒化物，选择三个参数来优化硒-Euryale ferox Salisb的合成工艺。Box-Behnken 设计的多糖 (Se-ESPS)。此外，从ESPS分离的ESPS-B1通过最佳合成工艺硒化为Se-ESPS-B1，然后探索了Se-ESPS-B1的表征和体外抗氧化活性。结果表明，按照 Se-ESPS 的最佳合成工艺（反应时间 5 h，反应温度 81 ℃，Na2SeO3 与 ESPS 的重量比为0.9 克/克）。一系列检测结果表明，Se-ESPS-B1 的表征与 ESPS-B1 的表征明显不同。而且，体外抗氧化实验表明，Se-ESPS-B1可通过清除DPPH、ABTS、·OH和增加还原能力发挥抗氧化活性。总之，合成过程是收获硒多糖的有效方法，Se-ESPS-B1 可用作潜在的抗氧化剂或硒营养补充剂。