Qing Jin Hua Tan Tang (QJHTT) exerts therapeutic effects in patients with chronic obstructive pulmonary disease (COPD) by alleviating inflammation. However, the anti-inflammatory components of QJHTT have not yet been reported. Our study aimed to screen the active anti-inflammatory components of QJHTT using a multivariate statistical analysis approach for spectrum-effect relationships. Different polar fractions of QJHTT were prepared using ethanol, ethyl acetate, and n-butanol to analyze the phytochemical components. Phytochemical fingerprints were generated using ultrahigh-performance liquid chromatography. In total, 24 peaks were observed in ten batches of QJHTT extracts. The anti-inflammatory activity was evaluated using a xylene-induced ear-swelling mouse model. Additionally, the spectrum-effect relationship between the relative areas of the 24 peaks and pharmacological activity was investigated using multivariate statistical analysis. The potential anti-inflammatory ingredients obtained from the screening (multivariate statistical analysis) will be validated for their anti-inflammatory effects and mechanisms utilizing a lipopolysaccharide-induced macrophage inflammation model. QJHTT ethanol extract 1 exhibited good anti-inflammatory activity. Peaks 11, 12, 13, 14, and 16, which were closely correlated with anti-inflammatory activity, were identified as meranzin, baicalin, baicalein, chrysin-7-O-β-D-glucuronide, and wogonoside, respectively. The anti-inflammatory activities of meranzin, baicalin, baicalein, and wogonoside were verified in vitro. These four bioactive components significantly inhibited the secretion of inflammatory factors in the lipopolysaccharide-stimulated macrophage cell line. This research successfully screened the QJHTT anti-inflammatory active ingredient group. Meranzin, baicalin, baicalein, chrysin-7-O-β-D-glucuronide, and wogonoside were predicted to be the anti-inflammatory active ingredient groups of QJHTT.

中文翻译：

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多因素谱效关系统计分析方法筛选秦金化痰汤抗炎成分

清津化痰汤（QJHTT）通过减轻炎症对慢性阻塞性肺疾病（COPD）患者发挥治疗作用。然而，QJHTT的抗炎成分尚未见报道。我们的研究旨在使用光谱效应关系的多变量统计分析方法筛选 QJHTT 的活性抗炎成分。使用乙醇、乙酸乙酯和n制备 QJHTT 的不同极性组分-丁醇来分析植物化学成分。使用超高效液相色谱法生成植物化学指纹。在 10 个批次的 QJHTT 提取物中总共观察到 24 个峰。使用二甲苯诱导的耳肿胀小鼠模型评估抗炎活性。此外，使用多变量统计分析研究了 24 个峰的相对面积与药理活性之间的谱效应关系。从筛选（多变量统计分析）中获得的潜在抗炎成分将利用脂多糖诱导的巨噬细胞炎症模型验证其抗炎作用和机制。QJHTT乙醇提取物1表现出良好的抗炎活性。峰 11、12、13、14 和 16，分别为 β -D-葡萄糖醛酸苷和黄芩苷。体外验证了美兰津、黄芩苷、黄芩素和汉黄芩苷的抗炎活性。这四种生物活性成分显着抑制了脂多糖刺激的巨噬细胞系中炎症因子的分泌。本次研究成功筛选了QJHTT抗炎活性成分组。Meranzin、黄芩苷、黄芩素、chrysin-7-O- β -D-glucuronide 和 wogonoside 被预测为 QJHTT 的抗炎活性成分组。