For clinical applications of cell-based therapies, a large quantity of human pluripotent stem cells (hPSCs) produced in standardized and scalable culture processes is required. Currently, microcarrier-free suspension culture shows potential for large-scale expansion of hPSCs; however, hPSCs tend to aggregate during culturing leading to a negative effect on cell yield. To overcome this problem, we developed a novel protocol to effectively control the sizes of cell aggregates and enhance the cell proliferation during the expansion of hPSCs in suspension.

中文翻译：

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硫酸葡聚糖和聚乙烯醇的组合防止过度聚集并促进多能干细胞在悬浮培养中的增殖

对于基于细胞的疗法的临床应用，需要在标准化和可扩展的培养过程中产生大量的人类多能干细胞 (hPSC)。目前，无微载体悬浮培养显示出 hPSC 大规模扩增的潜力；然而，hPSC 在培养过程中往往会聚集，从而对细胞产量产生负面影响。为了克服这个问题，我们开发了一种新的协议来有效控制细胞聚集体的大小，并在悬浮 hPSC 的扩增过程中增强细胞增殖。