Significance: Diffuse optical spectroscopic imaging (DOSI) is a versatile technology sensitive to changes in tissue composition and hemodynamics and has been used for a wide variety of clinical applications. Specific applications have prompted the development of versions of the DOSI technology to fit specific clinical needs. This work describes the development and characterization of a multi-modal DOSI (MM-DOSI) system that can acquire metabolic, compositional, and pulsatile information at multiple penetration depths in a single hardware platform. Additionally, a 3D tracking system is integrated with MM-DOSI, which enables registration of the acquired data to the physical imaging area. Aim: We demonstrate imaging, layered compositional analysis, and metabolism tracking capabilities using a single MM-DOSI system on optical phantoms as well as in vivo human tissue. Approach: We characterize system performance with a silicone phantom containing an embedded object. To demonstrate multi-layer sensitivity, we imaged human calf tissue with a 4.8-mm skin-adipose thickness. Human thenar tissue was also measured using a combined broadband DOSI and continuous-wave near-infrared spectroscopy method (∼15 Hz acquisition rate). Results: High-resolution optical property maps of absorption (μa) and reduced scattering (μs ′ ) were recovered on the phantom by capturing over 1000 measurement points in under 5 minutes. On human calf tissue, we show two probing depth layers have significantly different (p < 0.001) total-hemo/myoglobin and μs ′ composition. On thenar tissue, we calculate tissue arterial oxygen saturation, venous oxygen saturation, and tissue metabolic rate of oxygen consumption during baseline and after release of an arterial occlusion. Conclusions: The MM-DOSI can switch between collection of broadband spectra, high-resolution images, or multi-depth hemodynamics without any hardware reconfiguration. We conclude that MM-DOSI enables acquisition of high resolution, multi-modal data consolidated in a single platform, which can provide a more comprehensive understanding of tissue hemodynamics and composition for a wide range of clinical applications.

中文翻译：

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用于组织光学特性和血液动力学的高速监测和广域映射的多模态漫反射光谱学

意义：漫反射光谱成像 (DOSI) 是一种对组织成分和血流动力学变化敏感的通用技术，已被广泛用于各种临床应用。特定的应用程序促进了 DOSI 技术版本的开发，以满足特定的临床需求。这项工作描述了多模式 DOSI (MM-DOSI) 系统的开发和表征，该系统可以在单个硬件平台中获取多个穿透深度的代谢、成分和脉动信息。此外，3D 跟踪系统与 MM-DOSI 集成在一起，可以将采集的数据注册到物理成像区域。目标：我们展示成像、分层成分分析、使用单个 MM-DOSI 系统对光学体模和体内人体组织进行代谢跟踪能力。方法：我们使用包含嵌入对象的硅胶模型来表征系统性能。为了证明多层灵敏度，我们对具有 4.8 毫米皮肤脂肪厚度的人小腿组织进行了成像。还使用组合宽带 DOSI 和连续波近红外光谱法（~15 Hz 采集率）测量了人类鱼际组织。结果：通过在 5 分钟内捕获 1000 多个测量点，在体模上恢复了吸收 (μa) 和减少散射 (μs ' ) 的高分辨率光学特性图。在人类小腿组织上，我们显示两个探测深度层具有显着不同 (p < 0.001) 的总血/肌红蛋白和 μs ' 组成。在鱼际组织上，我们计算了基线期间和动脉闭塞解除后的组织动脉血氧饱和度、静脉血氧饱和度和氧消耗的组织代谢率。结论：MM-DOSI 可以在宽带光谱采集、高分辨率图像或多深度血流动力学之间切换，无需任何硬件重新配置。我们得出的结论是，MM-DOSI 能够在单个平台中获取合并的高分辨率、多模态数据，这可以为广泛的临床应用提供对组织血流动力学和成分的更全面的了解。或多深度血液动力学，无需任何硬件重新配置。我们得出的结论是，MM-DOSI 能够在单个平台中获取合并的高分辨率、多模态数据，这可以为广泛的临床应用提供对组织血流动力学和成分的更全面的了解。或多深度血液动力学，无需任何硬件重新配置。我们得出的结论是，MM-DOSI 能够在单个平台中获取合并的高分辨率、多模态数据，这可以为广泛的临床应用提供对组织血流动力学和成分的更全面的了解。