Refrigeration is an important method to extend shelf life of hardy kiwifruit. However, the inappropriate storage temperature can lead to chilling injury in the fruit. We found that firmness, total soluble solids, and total polyphenolic content of the fruit exposed to 0℃ environment were apparently lower, and titratable acidity content, browning rate, weight loss rate, electrolyte leakage, proline content, and malondialdehyde content were higher obviously than 4℃. A total of 244 differentially expressed proteins were found result from differential temperatures, among which 113 were up-regulated and 131 were down-regulated. Subcellular localization results presented that the differentially expressed proteins which were affected by low temperature were located in cytoplasmic, chloroplast, nuclear, mitochondrial, plasma membrane, and extracellular. Kyoto Encyclopedia of Genes and Genomes analysis showed that the differentially expressed proteins were mainly participated in synthesis of citrate cycle, oxidative phosphorylation, fatty acid biosynthesis, and starch and sucrose metabolism. Protein–protein interaction results revealed that central proteins interaction points respectively are 30S ribosomal proteins, 30S ribosomal protein S7, chloroplastic, cell division cycle 5-like protein, 50S ribosomal protein, ribosomal protein, ribosomal protein L6 protein, and SRP54 subunit protein. The quality deviations of all identified peptides were mainly distributed within 10 ppm, and MS2 has an ideal andromeda score, with more than 87.82% peptide scores above 60 points, and the median peptide score of 99.28 points. Therefore, the results of this study provide important information for new gene revelation and gene interaction relationship in hardy kiwifruit of chilling injury.

中文翻译：

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无标记技术蛋白质组揭示耐寒猕猴桃（Actinidia arguta）冷害机制

冷藏是延长耐寒猕猴桃保质期的重要方法。然而，不适当的储存温度会导致果实冷害。我们发现0℃环境下果实的硬度、总可溶性固形物和总多酚含量明显低于0℃，可滴定酸含量、褐变率、失重率、电解质渗漏率、脯氨酸含量、丙二醛含量明显高于0℃环境。 4℃。共发现244个差异表达蛋白，其中113个上调，131个下调。亚细胞定位结果表明，受低温影响的差异表达蛋白位于细胞质、叶绿体、细胞核、线粒体、质膜和细胞外。京都基因与基因组百科全书分析表明，差异表达蛋白主要参与柠檬酸循环合成、氧化磷酸化、脂肪酸生物合成以及淀粉和蔗糖代谢。蛋白-蛋白相互作用结果显示，中心蛋白相互作用点分别为30S核糖体蛋白、30S核糖体蛋白S7、叶绿体、细胞分裂周期5样蛋白、50S核糖体蛋白、核糖体蛋白、核糖体蛋白L6蛋白和SRP54亚基蛋白。所有鉴定肽段的质量偏差主要分布在10 ppm以内，MS2具有理想的仙女座分数，超过87.82%的肽段分数在60分以上，肽段分数中位数为99.28分。所以，