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Evaluation of the phytotoxicity of nano-particles on mung beans by internal extractive electrospray ionization mass spectrometry
Analyst ( IF 3.6 ) Pub Date : 2021-07-30 , DOI: 10.1039/d1an00871d
Haiyan Lu 1 , Hua Zhang 1 , Wei Zhou 2 , Huanwen Chen 2
Affiliation  

The wide application of nano-particles (NPs) has raised a serious concern over their impact on plants. However, evaluation of the effects of NPs on plant metabolism by direct detection of chemicals inside solid tissues presents a challenge. In this study, we report on a direct ionization method in mass spectrometry, internal extractive electrospray ionization (iEESI), for the direct evaluation of phytotoxicity of three different NPs (including CdTe quantum dots (CdTe QDs), gold nano-particles (Au NPs), and silver nano-particles (Ag NPs)) both on surfaces and inside solid tissues from the mung bean seeds (Vigna radiata) that were cultured in aqueous solutions of three NPs at 50 μg mL−1. The results showed that NPs could stimulate the biological accumulation of trigonelline and the decomposition of polysaccharides/oligosaccharides to glucose and maltose within 21 h of culture. To the best of our knowledge, this is the first study to apply internal extractive electrospray ionization mass spectrometry (iEESI-MS) for the direct measurement of solid tissue samples to evaluate the phytotoxicity of NPs on mung bean sprouts. Our study lays a solid foundation for further examination of other NPs-induced damaging effects such as apoptosis/necrosis, helping us to understand the phytotoxicity of NPs on plants.

中文翻译:

内萃取电喷雾电离质谱法评价纳米颗粒对绿豆的植物毒性

纳米粒子 (NPs) 的广泛应用引起了人们对其对植物影响的严重关注。然而,通过直接检测固体组织内的化学物质来评估 NPs 对植物代谢的影响是一个挑战。在这项研究中,我们报告了质谱中的直接电离方法,内部提取电喷雾电离 (iEESI),用于直接评估三种不同 NPs(包括 CdTe 量子点(CdTe QDs)、金纳米颗粒(Au NPs)的植物毒性) 和银纳米颗粒 (Ag NPs)) 表面和内部固体组织的绿豆种子(Vigna radiata),这些种子在 50 μg mL -1的三种纳米颗粒的水溶液中培养. 结果表明,NPs 可以刺激葫芦巴碱的生物积累和多糖/寡糖在培养 21 小时内分解为葡萄糖和麦芽糖。据我们所知,这是第一项应用内部萃取电喷雾电离质谱 (iEESI-MS) 直接测量固体组织样品以评估 NPs 对绿豆芽的植物毒性的研究。我们的研究为进一步检查其他 NPs 诱导的损伤效应(如细胞凋亡/坏死)奠定了坚实的基础,帮助我们了解 NPs 对植物的植物毒性。
更新日期:2021-08-15
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