In this research study, novel hydrogel composite films were constructed using different ratios of poly (vinyl alcohol) (PVA)/kappa-carrageenan (KC) (PVA90/KC10%, PVA80/KC20%, PVA70/KC30%, PVA60/KC40%) crosslinked with glutaraldehyde (0.025%) and investigated their physicochemical characteristics such as mechanical, thermal, morphological, swelling behaviour, and cell viability. SEM and FTIR revealed that surface morphology changed to heterogeneous and the presence of molecular interaction among the polymers. PVA90KC10 and PVA60KC40 exhibited smaller and larger pores on surface respectively. The change in the proportion of PVA and KC also triggered the tensile strength (Ts) of the film and the highest Ts observed were 21.60 MPa for PVA60KC40. Moreover, the thermal analysis showed three-phase degradation, and an increase in KC40 concentration results inversely proportional to a decrease in the rate of thermal degradation. Further, swelling and in-vitro biodegradation study confirmed the enhanced perseverance of water uptake for PVA60KC40 (286%) due to pores structure of the hydrogel film and PVA and KC alone degraded faster as compare to other films results suggested higher concentration of PVA90KC10 showed lower degradation rate and highest for PVA60KC40 about 6% and 22% respectively. Further, the cell viability was studied with MTT assay method by using NIH3T3 and HEK-293 cells for biocompatibility study revealed NIH3T3 cells were more biocompatible than HEK-293 and cell viability percent for PVA60KC40 showed the highest cell attachment about 99%. Overall corroborating data obtained from the study attested to the average swelling, appreciable mechanical characters, good interaction between molecules, and cell viability of the constructed PVA/KC hydrogel film, these all characters pave to be used as a potential template for biomedical applications such as tissue engineering and drug delivery.

在这项研究中，使用不同比例的聚乙烯醇（PVA）/κ-卡拉胶（KC）（PVA90/KC10%、PVA80/KC20%、PVA70/KC30%、PVA60/KC40%）构建了新型水凝胶复合膜) 与戊二醛 (0.025%) 交联，并研究了它们的物理化学特性，如机械、热、形态、膨胀行为和细胞活力。SEM 和 FTIR 显示表面形态发生变化，聚合物之间存在分子相互作用。PVA90KC10 和 PVA60KC40 分别在表面表现出越来越小的孔隙。PVA 和 KC 比例的变化也引发了薄膜的拉伸强度 (Ts)，观察到的最高 Ts 为 PVA60KC40 的 21.60 MPa。此外，热分析显示三相降解，KC40 浓度的增加与热降解速率的降低成反比。此外，肿胀和体外生物降解研究证实，由于水凝胶薄膜的孔隙结构，PVA60KC40 的吸水持久性增强（286%），与其他薄膜相比，单独的 PVA 和 KC 降解速度更快分别为 6% 和 22%。此外，通过使用 NIH3T3 和 HEK-293 细胞进行生物相容性研究，用 MTT 测定法研究细胞活力，发现 NIH3T3 细胞比 HEK-293 更具生物相容性，PVA60KC40 的细胞活力百分比显示最高的细胞附着率约为 99%。从研究中获得的总体确证数据证明了所构建的 PVA/KC 水凝胶膜的平均溶胀、明显的机械特性、良好的分子间相互作用和细胞活力，