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Biological Insights and NMR Metabolic Profiling of Different Extracts of Spermacoce verticillata (L.) G. Mey.
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-08-13 , DOI: 10.1002/cbdv.202100371 Kouadio Ibrahime Sinan 1 , Gokhan Zengin 1 , Antonio Fiorentino 2 , Brigida D'Abrosca 2 , Gunes Ak 1 , Devina Lobine 3 , Ouattara Katinan Etienne 4 , Anwar Hussein Subratty 3 , Mohamad Fawzi Mahomoodally 3
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-08-13 , DOI: 10.1002/cbdv.202100371 Kouadio Ibrahime Sinan 1 , Gokhan Zengin 1 , Antonio Fiorentino 2 , Brigida D'Abrosca 2 , Gunes Ak 1 , Devina Lobine 3 , Ouattara Katinan Etienne 4 , Anwar Hussein Subratty 3 , Mohamad Fawzi Mahomoodally 3
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Spermacoce verticillata (L.) G. Mey. is commonly used in the folk medicine by various cultures to manage common diseases. Herein, the chemical and biological profiles of S. verticillata were studied in order to provide a comprehensive characterization of bioactive compounds and also to highlight the therapeutic properties. The in vitro antioxidant activity using free-radical scavenging, phosphomolybdenum, ferrous-ion chelating and reducing power assays, and the inhibitory activity against key enzymes such as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase of S. verticillata extracts (dichloromethane, ethyl acetate, methanol and water) were investigated. The highest total phenolic and flavonoid content were observed in the methanolic and aqueous extracts. Exhaustive 2DNMR investigation has revealed the presence of rutin, ursolic and oleanoic acids. The methanolic extract, followed by aqueous extract have showed remarkable free radical quenching and reducing ability, while the dichloromethane extract was the best source of metal chelators. The tested extracts showed notable inhibitory activity against cholinesterases (AChE: 1.63–4.99 mg GALAE/g extract and BChE: 12.40–15.48 mg GALAE/g extract) and tyrosinase (60.85–159.64 mg KAE/g extract). No inhibitory activity was displayed by ethyl acetate and aqueous extracts against BChE and tyrosinase, respectively. All the tested extracts showed modest α-amylase inhibitory activity, while only the ethyl acetate and aqueous extracts were potent against α-glycosidase. This study further validates the use of S. verticillata in the traditional medicine, while advocating for further investigation for phytomedicine development.
中文翻译：
Spermacoce verticillata (L.) G. Mey 不同提取物的生物学见解和 NMR 代谢分析。
Spermacoce verticillata (L.) G. Mey。各种文化在民间医学中常用来治疗常见疾病。在此，研究了轮枝菌的化学和生物学特征，以提供生物活性化合物的综合表征，并突出其治疗特性。在体外使用自由基清除，磷钼，铁离子螯合和还原力测定法，和对关键酶，如乙酰胆碱酯酶和丁酰胆碱酯酶（BChE的），酪氨酸酶，α淀粉酶和α葡萄糖苷酶的抑制活性的抗氧化活性的S.藻研究了萃取物（二氯甲烷、乙酸乙酯、甲醇和水）。在甲醇和水提取物中观察到最高的总酚类和类黄酮含量。详尽的 2DNMR 调查揭示了芦丁、熊果酸和齐墩果酸的存在。甲醇提取物和水提取物显示出显着的自由基淬灭和还原能力，而二氯甲烷提取物是金属螯合剂的最佳来源。测试的提取物对胆碱酯酶（AChE：1.63–4.99 mg GALAE/g 提取物和 BChE：12.40–15.48 mg GALAE/g 提取物）和酪氨酸酶（60.85–159.64 mg KAE/g 提取物）显示出显着的抑制活性。乙酸乙酯和水提取物分别对 BChE 和酪氨酸酶没有显示抑制活性。所有测试的提取物都显示出适度的 α-淀粉酶抑制活性，而只有乙酸乙酯和水提取物对 α-糖苷酶有效。本研究进一步验证了使用S. verticillata在传统医学中，同时提倡进一步研究植物医学发展。
更新日期：2021-10-19
中文翻译：
Spermacoce verticillata (L.) G. Mey 不同提取物的生物学见解和 NMR 代谢分析。
Spermacoce verticillata (L.) G. Mey。各种文化在民间医学中常用来治疗常见疾病。在此，研究了轮枝菌的化学和生物学特征，以提供生物活性化合物的综合表征，并突出其治疗特性。在体外使用自由基清除，磷钼，铁离子螯合和还原力测定法，和对关键酶，如乙酰胆碱酯酶和丁酰胆碱酯酶（BChE的），酪氨酸酶，α淀粉酶和α葡萄糖苷酶的抑制活性的抗氧化活性的S.藻研究了萃取物（二氯甲烷、乙酸乙酯、甲醇和水）。在甲醇和水提取物中观察到最高的总酚类和类黄酮含量。详尽的 2DNMR 调查揭示了芦丁、熊果酸和齐墩果酸的存在。甲醇提取物和水提取物显示出显着的自由基淬灭和还原能力，而二氯甲烷提取物是金属螯合剂的最佳来源。测试的提取物对胆碱酯酶（AChE：1.63–4.99 mg GALAE/g 提取物和 BChE：12.40–15.48 mg GALAE/g 提取物）和酪氨酸酶（60.85–159.64 mg KAE/g 提取物）显示出显着的抑制活性。乙酸乙酯和水提取物分别对 BChE 和酪氨酸酶没有显示抑制活性。所有测试的提取物都显示出适度的 α-淀粉酶抑制活性，而只有乙酸乙酯和水提取物对 α-糖苷酶有效。本研究进一步验证了使用S. verticillata在传统医学中，同时提倡进一步研究植物医学发展。
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