A new perfusion mode of culture for WJ-MSCs expansion in a stirred and online monitored bioreactor,Biotechnology and Bioengineering

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A new perfusion mode of culture for WJ-MSCs expansion in a stirred and online monitored bioreactor
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2021-08-13 , DOI: 10.1002/bit.27914
Caroline Sion ₁ , Dima Ghannoum ₂ , Bruno Ebel ₁ , Fanny Gallo ₁ , Natalia de Isla ₂ , Emmanuel Guedon ₁ , Isabelle Chevalot ₁ , Eric Olmos ₁

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As a clinical dose requires a minimum of 10⁶ cells per kilogram of patients, it is, therefore, crucial to develop a scalable method of production of Wharton Jelly mesenchymal stem cells (WJ-MSCs) with maintained inner characteristics. Scalable expansion of WJ-MSCs on microcarriers usually found in cell culture, involves specific cell detachment using trypsin and could have harmful effects on cells. In this study, the performance of batch, fed-batch, and perfused-continuous mode of culture were compared. The batch and fed-batch modes resulted in expansion factors of 5 and 43, respectively. The perfused-continuous mode strategy consisted of the implementation of a settling tube inside the bioreactor. The diameter of the tube was calculated to maintain microcarriers colonized by cells in the bioreactor whereas empty microcarriers (responsible for potentially damaging collisions) were removed, using a continuous flow rate based on MSCs physiological requirements. Thanks to this strategy, a maximal number of 800 million cells was obtained in a 1.5 L bioreactor in 10 days. Lastly, online dielectric spectroscopy was implemented in the bioreactor and indicated that cell growth could be monitored during the culture.

中文翻译：

WJ-MSCs 在搅拌和在线监测生物反应器中扩增的新灌注培养模式

作为临床剂量至少需要 10 ⁶因此，开发一种可扩展的生产 Wharton Jelly 间充质干细胞 (WJ-MSCs) 并保持内部特征的方法至关重要。WJ-MSC 在通常存在于细胞培养中的微载体上的可扩展扩增涉及使用胰蛋白酶进行特定细胞脱离，并且可能对细胞产生有害影响。在本研究中，比较了分批、补料分批和灌注连续培养模式的性能。分批和分批补料模式分别导致膨胀因子为 5 和 43。灌注连续模式策略包括在生物反应器内安装沉降管。计算管的直径以维持生物反应器中细胞定植的微载体，而去除空的微载体（负责潜在的破坏性碰撞），使用基于 MSCs 生理要求的连续流速。由于这种策略，在 10 天内在 1.5 L 生物反应器中获得了最多 8 亿个细胞。最后，在生物反应器中实施了在线介电光谱，表明可以在培养过程中监测细胞生长。

更新日期：2021-10-13

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