Geomicrobiology Journal ( IF 2.2 ) Pub Date : 2021-08-12 , DOI: 10.1080/01490451.2021.1951398 Marwa Eltarahony 1 , Sahar Zaki 1 , Ayman Kamal 1, 2 , Desouky Abd-El-Haleem 1
Abstract
This study deals with 16S-rDNA identified bacteria, Lysinibacillus sp., Raoultella sp., and Streptomyces sp. capable of precipitating CaCO3 through nitrate reduction aerobically and anaerobically. The produced CaCO3 crystals were analyzed using XRD, EDX, FTIR, TGA and SEM. The results showed that the carbonatogenic bacteria served as nucleation sites for CaCO3 precipitation with distinct variation in polymorph and morphology; reflecting strain-specific property. Notably, the amount of precipitated CaCO3 recorded 3.27 (aerobic), 1.55 (anaerobic), 4.15 (aerobic), 3.75 (aerobic) and 1.87 (anaerobic) g/100 mL of strains Lysinibacillus sp., Streptomyces sp. and Raoultella sp., respectively, after 240 h of incubation. The study of changes in media chemistry during carbonatogenesis process revealed positive correlation between bacterial growth, nitrate reductase activity, pH, EC, Ca2+ depletion, amount of deposited CaCO3 and NO3− consumption. Therefore, the applications of these bacterial strains, which were employed for the first time in carbonatogenesis process, are promising in the environmental, biomedical and civil engineering fields.
中文翻译:
有氧和厌氧条件下碳化过程中硝酸盐异化细菌的方解石和球霰石生物合成
摘要
本研究涉及经 16S-rDNA 鉴定的细菌Lysinibacillus sp。, Raoultella sp. 和链霉菌属。能够通过有氧和无氧还原硝酸盐来沉淀CaCO 3。使用XRD、EDX、FTIR、TGA和SEM分析产生的CaCO 3晶体。结果表明,碳酸化细菌作为CaCO 3沉淀的成核位点,其多晶型和形态变化明显;反映应变特异性。值得注意的是,沉淀的 CaCO 3的量记录为 3.27(需氧)、1.55(厌氧)、4.15(需氧)、3.75(需氧)和 1.87(厌氧)g/100 mL赖氨酸杆菌属菌种。,链霉菌属。和Raoultella sp。,分别在孵育 240 小时后。对碳酸生成过程中培养基化学变化的研究揭示了细菌生长、硝酸盐还原酶活性、pH、EC、Ca 2+消耗、沉积的CaCO 3量和NO 3 -消耗之间的正相关。因此,这些首次用于碳生成过程的菌株在环境、生物医学和土木工程领域的应用前景广阔。