Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A hand-powered microfluidic system for portable and low-waste sample discretization
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-08-05 , DOI: 10.1039/d1lc00448d Tengbao Xie 1 , Ping Wang 2 , Lei Wu 3 , Bangyong Sun 1 , Qiang Zhao 1 , Gang Li 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-08-05 , DOI: 10.1039/d1lc00448d Tengbao Xie 1 , Ping Wang 2 , Lei Wu 3 , Bangyong Sun 1 , Qiang Zhao 1 , Gang Li 1
Affiliation
|
In this work, we present a simple and equipment-free system for discretizing samples into tens of thousands of discrete volumes in tens of seconds. Unlike conventional sample discretization systems that require bulky syringe pumps, pressure controllers, or vacuum equipment, our system requires only a sheet of water-soluble film, a hand-operated syringe, and a microfluidic device containing a high-density microchamber array. In this system, the water-soluble film seals the device inlet to form a closed channel–chamber system, while the syringe is used to create a vacuum in the closed system. Benefitting from the high negative pressure created by syringe-vacuum and the dissolution-triggered gating mechanism of the sealing water-soluble film, the aqueous sample loaded into the device inlet can be rapidly partitioned into tens of thousands of isolated chambers without the need for any expensive pumping systems. We demonstrated the utility of this system by exploiting it for digital PCR. We believe that this simple discretization system will find broad applications, such as in digital bioassays, single-cell analysis, and point-of-care diagnostics.
中文翻译：
用于便携式和低浪费样品离散化的手动微流控系统
在这项工作中，我们提出了一个简单且无需设备的系统，可在数十秒内将样本离散化为数万个离散体积。与需要笨重的注射泵、压力控制器或真空设备的传统样品离散化系统不同，我们的系统只需要一张水溶性薄膜、一个手动注射器和一个包含高密度微腔阵列的微流体装置。在该系统中，水溶性薄膜密封装置入口以形成封闭的通道-腔室系统，而注射器用于在封闭系统中产生真空。受益于注射器真空产生的高负压和密封水溶性薄膜的溶解触发门控机制，加载到设备入口的水样可以快速分隔到数以万计的隔离室中，而无需任何昂贵的泵送系统。我们通过将其用于数字 PCR 来展示该系统的实用性。我们相信，这种简单的离散化系统将获得广泛的应用，例如数字生物测定、单细胞分析和即时诊断。
更新日期：2021-08-13
中文翻译：
用于便携式和低浪费样品离散化的手动微流控系统
在这项工作中，我们提出了一个简单且无需设备的系统，可在数十秒内将样本离散化为数万个离散体积。与需要笨重的注射泵、压力控制器或真空设备的传统样品离散化系统不同，我们的系统只需要一张水溶性薄膜、一个手动注射器和一个包含高密度微腔阵列的微流体装置。在该系统中，水溶性薄膜密封装置入口以形成封闭的通道-腔室系统，而注射器用于在封闭系统中产生真空。受益于注射器真空产生的高负压和密封水溶性薄膜的溶解触发门控机制，加载到设备入口的水样可以快速分隔到数以万计的隔离室中，而无需任何昂贵的泵送系统。我们通过将其用于数字 PCR 来展示该系统的实用性。我们相信，这种简单的离散化系统将获得广泛的应用，例如数字生物测定、单细胞分析和即时诊断。
京公网安备 11010802027423号