Background: Due to the limitation of conventional cancer treatment using chemotherapy, the nanoparticle therapeutics have shown enhanced efficacy with alleviating side effects.

Objective: The aim of this study was to prepare the superparamagnetic iron oxide nanoparticles (TC- SPION) for doxorubicin (DOX) loading and delivery.

Methods: Here, we reported a simple green strategy to fabricate T-C-SPION using green tea extract and citric acid. Also, the anti-cancer drug, DOX, was used as a model drug to fabricate DOX-loaded nanoparticles.

Results: The formed T-C-SPION nanoparticles were spherical with a diameter of 23.8 ± 0.8 nm, as confirmed by Transmission Electron Microscopy (TEM). Besides, Dynamic Light Scattering (DLS) revealed that the prepared nanoparticles were water-dispersible and stable while stored in water for 6 weeks. The CCK-8 assay showed T-C-SPION to have a good cytocompatibility using different iron concentrations (10 ~ 120 ug/mL). Furthermore, T-C-SPION had a higher DOX encapsulation efficiency (E_encaps), around 43.2 ± 1.8 %, which resulted in a lagged release profile of DOX, compared to other types of iron oxide nanoparticles using green tea or citric acid alone. Next, cell viability assay indicated that T-C-SPION with a higher E_encaps showed superior and sustained cytotoxicity compared to the control group.

Conclusion: The developed iron oxide nanoparticles synthesized by green tea extract and citric acid in this paper could be considered as a potential drug carrier for cancer therapy applications.

背景：由于使用化学疗法进行常规癌症治疗的局限性，纳米颗粒疗法已显示出增强的疗效并减轻了副作用。

目的：本研究的目的是制备用于阿霉素 (DOX) 装载和递送的超顺磁性氧化铁纳米粒子 (TC-SPION)。

方法：在这里，我们报告了一种使用绿茶提取物和柠檬酸制造 TC-SPION 的简单绿色策略。此外，抗癌药物 DOX 被用作模型药物来制造负载 DOX 的纳米颗粒。

结果：如透射电子显微镜 (TEM) 所证实，形成的 TC-SPION 纳米颗粒是直径为 23.8 ± 0.8 nm 的球形。此外，动态光散射 (DLS) 表明，所制备的纳米粒子在水中储存 6 周时具有水分散性和稳定性。CCK-8 测定表明 TC-SPION 使用不同的铁浓度 (10 ~ 120 ug/mL) 具有良好的细胞相容性。此外，与单独使用绿茶或柠檬酸的其他类型的氧化铁纳米颗粒相比，TC-SPION 具有更高的 DOX 封装效率 (E _encaps )，约为 43.2 ± 1.8 %，这导致 DOX 的释放滞后。接下来，细胞活力测定表明具有更高 E 的 TC- _SPION 与对照组相比，显示出优异且持续的细胞毒性。

结论：本文开发的由绿茶提取物和柠檬酸合成的氧化铁纳米颗粒可被认为是用于癌症治疗应用的潜在药物载体。