Introduction: Substantial evidence has indicated that isoflurane leads to learning and memory impairment. This study was designed to investigate the potential role of microRNA-124-3p (miR-124-3p) in isoflurane-induced learning and memory impairment in rats. Methods: Spatial learning and memory of rats were estimated by the Morris water maze (MWM) test after the construction of isoflurane-treated models. qRT-PCR was performed to assess the expression levels of miR-124-3p. The levels of interleukin-1β, interleukin-6, and tumor necrosis factor-α in the hippocampal tissues were determined by enzyme-linked immunosorbent assay. The luciferase activity was determined by using a dual-luciferase reporter assay system. Results: The higher escape latency and lower time spent in the original quadrant were shown in isoflurane-treated rats compared with the control rats. Moreover, treatment with isoflurane could induce neuroinflammation, and miR-124-3p was poorly expressed in the hippocampal tissue of isoflurane-treated rats. Furthermore, STAT3 is a functional target of miR-124-3p, and inflammatory cytokine level was downregulated by miR-124-3p. Discussion/Conclusion: Combining the results of the current study demonstrates that miR-124-3p may have pivotal roles in improving isoflurane-induced learning and memory impairment via targeting STAT3 and inhibiting neuroinflammation.
Neuroimmunomodulation

中文翻译：

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miR-124-3p 通过靶向 STAT3 和抑制神经炎症改善异氟醚诱导的学习和记忆障碍

简介：大量证据表明异氟醚会导致学习和记忆障碍。本研究旨在研究 microRNA-124-3p (miR-124-3p) 在异氟醚诱导的大鼠学习和记忆障碍中的潜在作用。方法：构建异氟醚模型后，通过Morris水迷宫（MWM）试验评估大鼠的空间学习记忆能力。进行 qRT-PCR 以评估 miR-124-3p 的表达水平。酶联免疫吸附法测定海马组织中IL-1β、IL-6和肿瘤坏死因子-α的含量。通过使用双荧光素酶报告基因测定系统测定荧光素酶活性。结果：与对照大鼠相比，异氟醚治疗的大鼠显示出更高的逃逸潜伏期和在原始象限中花费的时间更少。此外，异氟醚治疗可诱导神经炎症，miR-124-3p在异氟醚治疗大鼠的海马组织中表达较差。此外，STAT3 是 miR-124-3p 的功能靶点，炎症细胞因子水平被 miR-124-3p 下调。讨论/结论：结合目前的研究结果表明，miR-124-3p 可能通过靶向 STAT3 和抑制神经炎症在改善异氟醚诱导的学习和记忆障碍方面发挥关键作用。
神经免疫调节