Introduction: Thyroid-stimulating hormone receptor (TSHR) is widely expressed in human tissues and cells. TSHR is not only involved in thyroid disease but also in the neuroendocrine-immune regulatory network. However, no study has exclusively focused on the expression and function of TSHR in natural killer (NK) cells. Methods: We studied TSHR expression using reverse transcription PCR to verify TSHR mRNA transcripts in human and mouse NK cells. Human and mouse thyroid and liver tissues as well as peripheral blood mononuclear cells (PBMCs) or spleen lymphoid cells (SLCs) were used as controls. The TSHR protein levels in NK-92 cells were determined by immunofluorescence staining. The function of TSHR in NK cells was investigated by measuring the TSH-stimulated cAMP levels. Results: TSHR mRNA was detected in human and mouse NK cells as well as in NK-92 cells and had the same sequence as that of thyroid-derived, PBMC-derived, and liver-derived mRNA. The TSHR protein was also expressed in the cell membrane of NK-92 cells. Furthermore, the cAMP levels in NK-92 cells were significantly higher after adding 10² mIU/mL of bovine TSH at p #x3c; 0.05, which stimulated cAMP production in NK-92 cells. Conclusions: Our findings confirm that TSHR is present and functional in NK cells and provide key clues for the potential regulatory effects of TSH on TSHR in NK cells in the immune system.
Neuroimmunomodulation

中文翻译：

---

NK细胞中TSHR的存在和TSH对NK细胞的作用

简介：促甲状腺激素受体（TSHR）在人体组织和细胞中广泛表达。TSHR 不仅参与甲状腺疾病，还参与神经内分泌-免疫调节网络。然而，没有研究专门关注 TSHR 在自然杀伤 (NK) 细胞中的表达和功能。方法：我们使用逆转录 PCR 研究了 TSHR 的表达，以验证人和小鼠 NK 细胞中的 TSHR mRNA 转录物。人类和小鼠甲状腺和肝脏组织以及外周血单个核细胞 (PBMC) 或脾淋巴细胞 (SLC) 用作对照。通过免疫荧光染色测定NK-92细胞中的TSHR蛋白水平。通过测量 TSH 刺激的 cAMP 水平来研究 TSHR 在 NK 细胞中的功能。结果：TSHR mRNA 在人和小鼠 NK 细胞以及 NK-92 细胞中检测到，并且与甲状腺来源、PBMC 来源和肝脏来源的 mRNA 具有相同的序列。TSHR蛋白也在NK-92细胞的细胞膜中表达。此外，在p #x3c加入 10 ² mIU/mL 牛 TSH后，NK-92 细胞中的 cAMP 水平显着升高；0.05，刺激 NK-92 细胞中 cAMP 的产生。结论：我们的研究结果证实 TSHR 在 NK 细胞中存在并发挥作用，并为 TSH 对免疫系统 NK 细胞中 TSHR 的潜在调节作用提供关键线索。神经免疫调节