Lignocellulosic by-products from agricultural crops represent an important raw material for anaerobic digestion and clean renewable, which is a key component of the circular economy. Lignocellulose is recalcitrant to biodegradation and pretreatments are required to increase methane yield during anaerobic digestion. In this work, the efficacy of different physicochemical pretreatments was compared using corn stover biomass as substrate. Anaerobic digestion of untreated and pretreated corn stover was performed in batch mode at mesophilic temperature (38°C) and organic matter solubilization of pretreated substrates was also investigated. The highest organic matter solubilization occurred in autoclave pretreatment (soluble chemical oxygen demand = 5630 ± 42 mg O₂ L⁻¹). However, the highest methane yield was obtained using alkaline pretreatment (367 ± 35 mL CH₄ g⁻¹ VS_added). Alkaline pretreatment increased methane yield by 43.3% compared to untreated control (256 ± 15 mL CH₄ g⁻¹ VS_added). Two mathematical models (i.e. first-order kinetics and transfer function) were utilized to fit the experimental data with the aim of assessing anaerobic biodegradation and to obtain the kinetic constants in all cases studied. Both models adequately fit the experimental results. The kinetic constant, k, of the first-order model increased by 92.8% when stover was pretreated with sulphuric acid compared with control. The transfer function model revealed that the maximum methane production rate, R_m, was obtained for the sulphuric acid treatment, which was 63.5% higher compared to control.

农作物的木质纤维素副产品是厌氧消化和清洁可再生的重要原料，是循环经济的关键组成部分。木质纤维素难以生物降解，需要预处理以增加厌氧消化过程中的甲烷产量。在这项工作中，以玉米秸秆生物质为基质，比较了不同物理化学预处理的效果。未处理和预处理的玉米秸秆的厌氧消化在中温温度（38°C）下以间歇模式进行，并且还研究了预处理底物的有机物溶解度。最高的有机物溶解发生在高压釜预处理（可溶性化学需氧量 = 5630 ± 42 mg O ₂  L ^-1）。然而，使用碱性预处理（_{添加367 ± 35 mL CH 4}  g ^-1  VS ）获得了最高的甲烷产率。与未处理的对照（添加 256 ± 15 mL CH ₄  g ^-1  VS ）相比，碱性预处理使甲烷产量_增加了 43.3% 。使用两个数学模型（即一级动力学和传递函数）来拟合实验数据，目的是评估厌氧生物降解并获得所有研究案例中的动力学常数。两种模型都充分拟合了实验结果。动力学常数，k, 与对照相比, 秸秆经硫酸预处理后, 一阶模型的 , 增加了 92.8%。传递函数模型显示，硫酸处理获得的最大甲烷产率R _{m比对照高 63.5%。}