Ten of 40 cows died within 48 h of gaining access to a barn in which various chemicals were stored. Some of the surviving cows exhibited drooling, muscle tremors, and agitation. Postmortem examinations of 2 cows were performed in the field, and revealed nonspecific, moderate-to-severe pulmonary congestion. Liver and rumen contents, each from a different cow, were analyzed using a qualitative, multi-residue GC-MS method validated for the detection of pesticides and other chemical analytes. Using this method, extracts from the liver and rumen content samples were compared to atrazine (neat standard) and matrix-matched, control samples fortified with atrazine. GC-MS analysis detected atrazine at 215 m/z (NIST match >97%) with a retention time of ~13 min in liver and rumen content samples from our case. Detection of atrazine in the samples from the cows in this herd, combined with the clinical history, indicate that atrazine toxicity was the likely cause of clinical signs and death observed in this herd.

40 头奶牛中有 10 头在进入存放各种化学品的谷仓后 48 小时内死亡。一些幸存的奶牛流口水、肌肉颤抖和激动。在野外对 2 头奶牛进行了尸检，结果显示非特异性、中度至重度肺充血。使用经过验证可用于检测农药和其他化学分析物的定性多残留 GC-MS 方法分析来自不同奶牛的肝脏和瘤胃内容物。使用该方法，将肝脏和瘤胃含量样品的提取物与阿特拉津（纯标准品）和基质匹配、添加了阿特拉津的对照样品进行比较。GC-MS 分析在我们案例的肝脏和瘤胃含量样品中检测到 215 m/z（NIST 匹配 >97%）的阿特拉津，保留时间约为 13 分钟。