当前位置：
X-MOL 学术
›
Mol. Plant Pathol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Southern rice black-streaked dwarf virus hijacks SNARE complex of its insect vector for its effective transmission to rice
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-08-13 , DOI: 10.1111/mpp.13109 Lu Zhang 1 , Wenwen Liu 1 , Xiaowan Zhang 1 , Li Li 1 , Xifeng Wang 1
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-08-13 , DOI: 10.1111/mpp.13109 Lu Zhang 1 , Wenwen Liu 1 , Xiaowan Zhang 1 , Li Li 1 , Xifeng Wang 1
Affiliation
|
Vesicular trafficking is an important dynamic process that facilitates intracellular transport of biological macromolecules and their release into the extracellular environment. However, little is known about whether or how plant viruses utilize intracellular vesicles to their advantage. Here, we report that southern rice black-streaked dwarf virus (SRBSDV) enters intracellular vesicles in epithelial cells of its insect vector by engaging VAMP7 and Vti1a proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex. The major outer capsid protein P10 of SRBSDV was shown to interact with VAMP7 and Vti1a of the white-backed planthopper and promote the fusion of vesicles into a large vesicle, which finally fused with the plasma membrane to release virions from midgut epithelial cells. Downregulation of the expression of either VAMP7 or Vti1a did not affect viral entry and accumulation in the gut, but significantly reduced viral accumulation in the haemolymph. It also did not affect virus acquisition, but significantly reduced the virus transmission efficiency to rice. Our data reveal a critical mechanism by which a plant reovirus hijacks the vesicle transport system to overcome the midgut escape barrier in vector insects and provide new insights into the role of the SNARE complex in viral transmission and the potential for developing novel strategies of viral disease control.
中文翻译：
南方水稻黑条矮缩病毒劫持其昆虫载体的 SNARE 复合物有效传播到水稻
囊泡运输是一个重要的动态过程，可促进生物大分子的细胞内运输及其释放到细胞外环境中。然而，关于植物病毒是否或如何利用细胞内囊泡来发挥其优势，我们知之甚少。在这里，我们报道南方水稻黑条矮缩病毒 (SRBSDV) 通过与可溶性N中的 VAMP7 和 Vti1a 蛋白结合，进入其昆虫载体上皮细胞的细胞内囊泡。-乙基马来酰亚胺敏感因子附着蛋白受体 (SNARE) 复合物。SRBSDV 的主要外衣壳蛋白 P10 与白背飞虱的 VAMP7 和 Vti1a 相互作用，促进囊泡融合成一个大囊泡，最终与质膜融合，从中肠上皮细胞释放病毒粒子。VAMP7 或 Vti1a 表达的下调不影响病毒在肠道中的进入和积累，但显着减少了血淋巴中的病毒积累。它也不影响病毒的获取，但显着降低了病毒对水稻的传播效率。
更新日期：2021-09-12
中文翻译：
南方水稻黑条矮缩病毒劫持其昆虫载体的 SNARE 复合物有效传播到水稻
囊泡运输是一个重要的动态过程，可促进生物大分子的细胞内运输及其释放到细胞外环境中。然而，关于植物病毒是否或如何利用细胞内囊泡来发挥其优势，我们知之甚少。在这里，我们报道南方水稻黑条矮缩病毒 (SRBSDV) 通过与可溶性N中的 VAMP7 和 Vti1a 蛋白结合，进入其昆虫载体上皮细胞的细胞内囊泡。-乙基马来酰亚胺敏感因子附着蛋白受体 (SNARE) 复合物。SRBSDV 的主要外衣壳蛋白 P10 与白背飞虱的 VAMP7 和 Vti1a 相互作用，促进囊泡融合成一个大囊泡，最终与质膜融合，从中肠上皮细胞释放病毒粒子。VAMP7 或 Vti1a 表达的下调不影响病毒在肠道中的进入和积累，但显着减少了血淋巴中的病毒积累。它也不影响病毒的获取，但显着降低了病毒对水稻的传播效率。
京公网安备 11010802027423号