Quality assurance: Evaluation and comparison of methods for electron microscopic measurement of GBM width and the effect of in-lab calibration in diagnostic renal pathology,Microscopy Research and Technique

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Quality assurance: Evaluation and comparison of methods for electron microscopic measurement of GBM width and the effect of in-lab calibration in diagnostic renal pathology
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-08-12 , DOI: 10.1002/jemt.23904
Meral Uner _{1,

2} , Secil Demirkol Canlı ₃ , Lois J Arend ₁

Affiliation

Replacing equipment and software can improve efficiency and allow updates to laboratory procedures, but has the potential to introduce changes in established values for a laboratory. Replacement of an electron microscope (EM), fitted with an updated digital camera, and use of new software for imaging and analysis prompted this QA study to ensure that new equipment, imaging, and measurement of the glomerular basement membrane (GBM) produced data consistent with the laboratory's established range of normal width. GBM measurements from 14 randomly selected human renal biopsies were compared using five different approaches. Original measurements of GBMs obtained on the laboratory's previous EM were compared to images collected on the new microscope with measurements performed using new software, as well as the original images and the new images measured using a separate software method as a control. The widths obtained by five approaches were compared to each other. While measurements showed minor variability between the approaches, significant difference in GBM width was noted in three of the paired comparisons. In some cases, these differences suggested slight diagnostic changes. Evaluation of new equipment, software, and techniques is important for a laboratory's quality assurance. While new equipment and/or procedures can introduce errors in test outcomes, we found that different EMs, cameras, and software made slight differences in our laboratory's values for kidney GBM width. However, a few cases showed enough difference in GBM width to suggest a change in diagnosis, illustrating the necessity of calibration adjustments in the setting of new equipment and software.

中文翻译：

质量保证：GBM 宽度的电子显微镜测量方法的评估和比较以及实验室校准在诊断肾脏病理学中的效果

更换设备和软件可以提高效率并允许更新实验室程序，但有可能改变实验室的既定值。更换电子显微镜 (EM)，配备更新的数码相机，并使用新的成像和分析软件，促使这项 QA 研究确保肾小球基底膜 (GBM) 的新设备、成像和测量产生一致的数据与实验室既定的正常宽度范围。使用五种不同的方法比较了来自 14 个随机选择的人肾活检的 GBM 测量值。在实验室之前的 EM 上获得的 GBM 的原始测量值与在新显微镜上收集的图像进行比较，并使用新软件进行测量，以及使用单独的软件方法测量的原始图像和新图像作为对照。通过五种方法获得的宽度相互比较。虽然测量结果显示方法之间的差异很小，但在三个配对比较中注意到 GBM 宽度的显着差异。在某些情况下，这些差异表明诊断略有变化。新设备、软件和技术的评估对于实验室的质量保证很重要。虽然新设备和/或程序可能会在测试结果中引入错误，但我们发现不同的 EM、相机和软件在我们实验室的肾脏 GBM 宽度值中略有不同。然而，少数病例的 GBM 宽度差异足以表明诊断的变化，

更新日期：2021-08-12

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