In this study, a c-type lysozyme gene (BsLyzC) was identified and characterized from a marine fish, Bostrychus sinensis. The BsLyzC encodes 154 amino acids and contains a signal peptide of 17 amino acids, two catalytic residues and eight cysteine residues. The genomic DNA of BsLyzC consists of four exons and three introns. The BsLyzC shares high sequence similarity with c-type lysozyme from other fish species. The qPCR assays indicated that the BsLyzC exhibited a constitutive expression pattern in eleven examined tissues of healthy B. sinensis individuals. The transcripts of BsLyzC could be significantly induced after infection of Vibrio parahemolyticus in blood, spleen and head kidney. The optimal temperature and pH for recombinant BsLyzC (rBsLyzC) were found to be 50 °C and 6.0, respectively. The rBsLyzC exhibited antibacterial activities against two Gram-positive bacteria and two Gram-negative bacteria. These results indicate that the BsLyzC is involved in the antibacterial immunity of B. sinensis.

在这项研究中，从一种海鱼Bostrychus sinensis中鉴定和表征了一个 c 型溶菌酶基因 (BsLyzC) 。BsLyzC 编码 154 个氨基酸，包含 17 个氨基酸、两个催化残基和八个半胱氨酸残基的信号肽。BsLyzC 的基因组 DNA 由四个外显子和三个内含子组成。BsLyzC 与来自其他鱼类的 c 型溶菌酶具有高度的序列相似性。qPCR 分析表明，BsLyzC 在健康的中华芽孢杆菌个体的 11 个检查组织中表现出组成型表达模式。副溶血性弧菌感染后可显着诱导BsLyzC转录本归血、脾、头肾。重组 BsLyzC (rBsLyzC) 的最佳温度和 pH 值分别为 50 °C 和 6.0。rBsLyzC 对两种革兰氏阳性菌和两种革兰氏阴性菌表现出抗菌活性。这些结果表明BsLyzC参与了中华芽孢杆菌的抗菌免疫。