The control of secondary metabolism in fungi is essential for the regulation of various cellular functions. In this study, we searched the RIKEN Natural Products Depository (NPDepo) chemical library for inducers of tenuazonic acid (TeA) production in the rice blast fungus Pyricularia oryzae and identified NPD938. NPD938 transcriptionally induced TeA production. We explored the mode of action of NPD938 and observed that this compound enhanced TeA production via LAE1, a global regulator of fungal secondary metabolism. NPD938 could also induce production of terpendoles and pyridoxatins in Tolypocladium album RK99-F33. Terpendole production was induced transcriptionally. We identified the pyridoxatin biosynthetic gene cluster among transcriptionally induced secondary metabolite biosynthetic gene clusters. Therefore, NPD938 is useful for the control of fungal secondary metabolism.

中文翻译：

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筛选诱导丁烯酸产生的化合物并鉴定 NPD938 作为真菌次生代谢的调节剂。


真菌次生代谢的控制对于调节各种细胞功能至关重要。在这项研究中，我们在 RIKEN 天然产物保藏库 (NPDepo) 化学库中检索了稻瘟病菌 Pyrularia oryzae 中产生 Tenuazonic Acid (TeA) 的诱导剂，并鉴定出 NPD938。 NPD938 转录诱导 TeA 产生。我们探索了 NPD938 的作用模式，并观察到该化合物通过 LAE1（真菌次生代谢的全局调节因子）增强 TeA 的产生。 NPD938 还可以诱导 Tolypocladium album RK99-F33 中萜烯和吡哆嗪的产生。萜烯的产生是通过转录诱导的。我们在转录诱导的次级代谢物生物合成基因簇中鉴定了吡哆沙汀生物合成基因簇。因此，NPD938可用于控制真菌次生代谢。