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Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I
Journal of Cell Biology ( IF 7.4 ) Pub Date : 2021-08-11 , DOI: 10.1083/jcb.202011153
Tianyu Wu 1 , Simon I R Lane 2 , Stephanie L Morgan 3 , Feng Tang 4 , Keith T Jones 5
Affiliation  

The repetitive sequences of DNA centromeric regions form the structural basis for kinetochore assembly. Recently they were found to be transcriptionally active in mitosis, with their RNAs providing noncoding functions. Here we explore the role, in mouse oocytes, of transcripts generated from within the minor satellite repeats. Depletion of minor satellite transcripts delayed progression through meiosis I by activation of the spindle assembly checkpoint. Arrested oocytes had poorly congressed chromosomes, and centromeres were frequently split by microtubules. Thus, we have demonstrated that the centromeric RNA plays a specific role in female meiosis I compared with mitosis and is required for maintaining the structural integrity of centromeres. This may contribute to the high aneuploidy rates observed in female meiosis.

中文翻译:

着丝粒 RNA 的丢失激活哺乳动物雌性减数分裂 I 中的纺锤体组装检查点

DNA 着丝粒区域的重复序列构成着丝粒组装的结构基础。最近发现它们在有丝分裂中具有转录活性,其 RNA 提供非编码功能。在这里,我们探讨了小卫星重复中产生的转录本在小鼠卵母细胞中的作用。通过激活纺锤体装配检查点,小卫星转录物的消耗延迟了减数分裂 I 的进展。停滞的卵母细胞染色体聚集不良,着丝粒经常被微管分裂。因此,我们证明,与有丝分裂相比,着丝粒 RNA 在雌性减数分裂 I 中发挥着特定作用,并且是维持着丝粒结构完整性所必需的。这可能导致女性减数分裂中观察到的高非整倍体率。
更新日期:2021-08-11
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