In much of the United States and Canada, the common reed consists of both a native subspecies Phragmites australis americanus, and a highly invasive introduced subspecies P. australis australis. DNA testing is generally used to distinguish them definitively and is necessary to detect hybridization. We report a group of single nucleotide polymorphisms and indels in the nuclear NRT2 gene of Phragmites that differentiate North American native and European-introduced populations. All native samples tested were identical in NRT2 sequence over 1564 bases except for two positions. There were nine positions, consisting of seven base substitutions and two indels, at which all introduced samples were fixed for a different allele than the native samples. For the two indels, samples collected from northern Europe were also fixed for the same allele as the introduced samples collected across North America. One of the indels was easily detected by a PCR-RFLP assay and provides a rapid and inexpensive way to screen for hybrids between native and European-introduced populations of Phragmites and thus can facilitate more widespread surveillance for hybrids between native and introduced populations in North America.

在美国和加拿大的大部分地区，普通芦苇由本地亚种Phragmites australis americanus和高度入侵的引入亚种P. australis australis 组成。DNA 测试通常用于明确区分它们，并且是检测杂交所必需的。我们报告了芦苇核NRT2基因中的一组单核苷酸多态性和插入缺失，这些基因区分了北美本地和欧洲引入的种群。在NRT2 中测试的所有原生样品都相同除两个位置外，序列超过 1564 个碱基。有 9 个位置，由 7 个碱基替换和 2 个插入缺失组成，所有引入的样本均针对与天然样本不同的等位基因进行固定。对于这两个插入缺失，从北欧收集的样本也固定为与在北美收集的引入样本相同的等位基因。其中一个插入缺失很容易通过 PCR-RFLP 测定法检测到，并提供了一种快速且廉价的方式来筛选本地和欧洲引入的芦苇种群之间的杂种，因此可以促进对北美本地和引入种群之间的杂种进行更广泛的监测.