Two genes encoding lytic polysaccharide monooxygenases (LPMOs) CsLPMO9A and CsLPMO9B were cloned from the white-rot fungus Ceriporiopsis subvermispora and successfully expressed in Komagataella phaffii. The oxidative degradation of phosphoric acid swollen cellulose by the LPMOs was conducted using ascorbic acid as an electron donor. Analysis with high performance anion-exchange chromatography and matrix assisted laser desorption/ionization-time-of-flight mass spectrometry indicated that the two enzymes cleaved the polysaccharides by oxidization of the C1 carbon atom of the glucose unit. Synergistic effects between CsLPMO9A or CsLPMO9B and a commercial cellulase cocktail from Trichoderma reesei were observed in the saccharification of delignified wheat straw. The amounts of glucose, cellobiose and xylose released from the substrate by the combined enzymes were increased by 83 %, 16 % and 47 % (for CsLPMO9A), or 79 %, 52 % and 81 % (for CsLPMO9B), compared to the cellulase alone, respectively. The two new enzymes displayed promising potential in industrial bioconversion of lignocellulosic biomass.

编码裂解多糖单加氧酶（LPMOs）两个基因铯LPMO9A和Cs的LPMO9B是从白腐菌克隆拟蜡菌属subvermispora和在成功表达Komagataella phaffii。使用抗坏血酸作为电子供体进行 LPMO 对磷酸溶胀纤维素的氧化降解。高效阴离子交换色谱和基质辅助激光解吸/电离飞行时间质谱分析表明，这两种酶通过氧化葡萄糖单元的 C1 碳原子来裂解多糖。Cs LPMO9A 或Cs LPMO9B 与商业纤维素酶混合物之间的协同作用在脱木质素麦秆的糖化过程中观察到里氏木霉。与酶相比，组合酶从底物释放的葡萄糖、纤维二糖和木糖的量分别增加了 83%、16% 和 47%（对于Cs LPMO9A），或 79%、52% 和 81%（对于Cs LPMO9B）单独的纤维素酶，分别。这两种新酶在木质纤维素生物质的工业生物转化中显示出有希望的潜力。