In-Situ Encapsulation of Protein into Nanoscale Hydrogen-Bonded Organic Frameworks for Intracellular Biocatalysis,Angewandte Chemie International Edition

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In-Situ Encapsulation of Protein into Nanoscale Hydrogen-Bonded Organic Frameworks for Intracellular Biocatalysis
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2021-08-12 , DOI: 10.1002/anie.202105634
Jiakang Tang _{1,

2} , Ji Liu _{1,

2} , Qizhen Zheng _{1,

2} , Wenting Li _{1,

2} , Jinhan Sheng _{1,

2} , Lanqun Mao ₃ , Ming Wang _{1,

2}

Affiliation

Hydrogen-bonded organic frameworks (HOFs) are porous materials with great potential for biological applications. The self-assembly of HOFs and biomacromolecules, however, is challenging. We report herein the self-assembly of nanoscale HOFs (nHOFs) to encapsulate protein for intracellular biocatalysis. The self-assembly of tetrakis(4-amidiniumphenyl)methane and azobenzenedicarboxylate can encapsulate protein in situ to form protein@nHOFs under mild conditions. This strategy is applicable to proteins with different surface charge and molecular weight, showing a high protein encapsulation efficiency and minimal effect on protein activity. A cellular delivery study shows that the protein@TA-HOFs can efficiently enter cells and retain enzyme activity for biochemical catalysis in living cells for neuroprotection. Our strategy paves new avenues for interfacing nHOFs with biological settings and sheds light on expanding nHOFs as a platform for biomacromolecule delivery and disease treatment.

中文翻译：

蛋白质原位封装成纳米级氢键有机框架用于细胞内生物催化

氢键有机骨架（HOFs）是一种多孔材料，具有巨大的生物应用潜力。然而，HOFs 和生物大分子的自组装具有挑战性。我们在此报告了纳米级 HOF（nHOF）的自组装以封装蛋白质用于细胞内生物催化。四（4-脒苯基）甲烷和偶氮苯二羧酸盐的自组装可以在温和条件下原位包裹蛋白质形成蛋白质@nHOFs。该策略适用于具有不同表面电荷和分子量的蛋白质，表现出较高的蛋白质包封效率和对蛋白质活性的最小影响。一项细胞递送研究表明，蛋白质@TA-HOFs 可以有效地进入细胞并保留酶活性，以在活细胞中进行生化催化，从而起到神经保护作用。

更新日期：2021-09-27

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