Purpose: miRNAs can act as oncogenes or tumor suppressors and participate in the development and progression of tumors, thus affecting the prognosis and survival of cancer patients. In this paper, we mainly studied the role of miR-1826 in prostate cancer.
Patients and Methods: The expression of miR-1826 was studied by quantitative real-time PCR (qRT-PCR). Kaplan–Meier curves were used to analyze the relationship between the expression of miR-1826 and the survival rate of PC patients. Cox regression analysis was used to study the risk factors affecting the prognosis of PC patients. PC cells were transfected with miR-1826 mimic, mimic negative control (mimic NC), miR-1826 inhibitor, or inhibitor NC. The effect of miR-1826 on the proliferation of PC cells was studied by the CCK-8 method and colony formation assay. Transwell assays were used to detect the effect of miR-1826 on the migratory and invasive abilities of tumor cells.
Results: The expression of miR-1826 in PC tissues was lower than that in adjacent normal tissues, and that the expression levels of miR-1826 in four PC cell lines were all lower than normal human prostate epithelial cell lines. Patients with low expression of miR-1826 had shorter overall survival compared with those with high expression. The downregulation of miR-1826 promoted PC cell proliferation, migration, and invasion.
Conclusion: In summary, the low expression of miR-1826 may promote the progression of PC, and the low expression of miR-1826 is also associated with a poor prognosis in PC patients.

Keywords: miR-1826, prostate cancer, prognostic value, progression, treatment


中文翻译：

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miR-1826在前列腺癌中的预后价值及其对肿瘤进展的调节作用

目的： miRNAs可以作为癌基因或抑癌基因，参与肿瘤的发生发展，从而影响癌症患者的预后和生存。本文主要研究了miR-1826在前列腺癌中的作用。
患者和方法：通过定量实时PCR（qRT-PCR）研究miR-1826的表达。Kaplan-Meier曲线用于分析miR-1826的表达与PC患者生存率的关系。Cox回归分析用于研究影响PC患者预后的危险因素。用 miR-1826 模拟物、模拟物阴性对照（模拟 NC）、miR-1826 抑制剂或抑制剂 NC 转染 PC 细胞。通过CCK-8法和集落形成试验研究了miR-1826对PC细胞增殖的影响。Transwell 检测用于检测 miR-1826 对肿瘤细胞迁移和侵袭能力的影响。
结果：miR-1826在PC组织中的表达低于邻近正常组织，4个PC细胞系中miR-1826的表达水平均低于正常人前列腺上皮细胞系。与高表达的患者相比，miR-1826低表达的患者总生存期较短。miR-1826的下调促进了PC细胞的增殖、迁移和侵袭。
结论：综上所述，miR-1826的低表达可能促进PC的进展，miR-1826的低表达也与PC患者预后不良有关。

关键词： miR-1826，前列腺癌，预后价值，进展，治疗