Abstract

Gloeostereum incarnatum has edible and medicinal value and was first cultivated and domesticated in China. We sequenced the G. incarnatum monokaryotic strain GiC-126 on an Illumina HiSeq X Ten system and obtained a 34.52-Mb genome assembly sequence that encoded 16,895 predicted genes. We combined the GiC-126 genome with the published genome of G. incarnatum strain CCMJ2665 to construct a genetic linkage map (GiC-126 genome) that had 10 linkage groups (LGs), and the 15 assembly sequences of CCMJ2665 were integrated into 8 LGs. We identified 1912 simple sequence repeat (SSR) loci and detected 700 genes containing 768 SSRs in the genome; 65 and 100 of them were annotated with gene ontology (GO) terms and KEGG pathways, respectively. Carbohydrate-active enzymes (CAZymes) were identified in 20 fungal genomes and annotated; among them, 144 CAZymes were annotated in the GiC-126 genome. The A mating-type locus (MAT-A) of G. incarnatum was located on scaffold885 at 38.9 cM of LG1 and was flanked by two homeodomain (HD1) genes, mip and beta-fg. Fourteen segregation distortion markers were detected in the genetic linkage map, all of which were skewed toward the parent GiC-126. They formed three segregation distortion regions (SDR1–SDR3), and 22 predictive genes were found in scaffold1920 where three segregation distortion markers were located in SDR1. In this study, we corrected and updated the genomic information of G. incarnatum. Our results will provide a theoretical basis for fine gene mapping, functional gene cloning, and genetic breeding the follow-up of G. incarnatum.

摘要

金银花具有食用和药用价值，在我国最早栽培驯化。我们在 Illumina HiSeq X Ten 系统上对G. incarnatum单核菌株 GiC-126 进行了测序，并获得了一个 34.52-Mb 的基因组组装序列，该序列编码了 16,895 个预测基因。我们将 GiC-126 基因组与已发表的G. incarnatum基因组相结合菌株CCMJ2665构建具有10个连锁群（LGs）的遗传连锁图谱（GiC-126基因组），将CCMJ2665的15个组装序列整合到8个LGs中。我们鉴定了 1912 个简单序列重复 (SSR) 位点，并在基因组中检测到 700 个基因，其中包含 768 个 SSR；其中 65 个和 100 个分别用基因本体 (GO) 术语和 KEGG 通路进行了注释。在 20 个真菌基因组中鉴定并注释了碳水化合物活性酶 (CAZymes)；其中，144个CAZymes在GiC-126基因组中被注释。G. incarnatum的 A 交配型基因座 ( MAT-A )位于 LG1 38.9 cM 处的 scaffold885 上，两侧是两个同源域 (HD1) 基因，mip和beta-fg. 在遗传连锁图中检测到 14 个分离扭曲标记，所有这些标记都向亲本 GiC-126 倾斜。他们形成了三个分离扭曲区域（SDR1-SDR3），在 scaffold1920 中发现了 22 个预测基因，其中三个分离扭曲标记位于 SDR1。在这项研究中，我们更正和更新了G. incarnatum的基因组信息。我们的研究结果将为G. incarnatum的后续精细基因定位、功能基因克隆和遗传育种提供理论依据。