Fibrin as an extracellular matrix feature like biocompatibility, creates a favorable environment for proliferation and migration of cells and it can act as a reservoir for storage and release of growth factors in tissue engineering. In this study, the inner surface of electrospun poly (lactic-co-glycolic acid) (PLGA) nanofibrous conduit was biofunctionalized with laminin containing brain derived neurotrophic factor (BDNF) and gold nanoparticles in chitosan nanoparticle. The rats were randomly divided into five groups, including autograft group as the positive control, PLGA conduit coated by laminin and filled with DMEM/F12, PLGA conduit coated by laminin and filled with rat-adipose derived stem cells (r-ADSCs), PLGA conduit coated by laminin containing gold-chitosan nanoparticles (AuNPs-CNPs), BDNF-chitosan nanoparticles (BDNF-CNPs) and filled with r-ADSCs or filled with r-ADSCs suspended in fibrin matrix, and they were implanted into a 10 mm rat sciatic nerve gap. Eventually, axonal regeneration and functional recovery were assessed after 12 weeks. After 3 months post-surgery period, the results showed that in the PLGA conduit filled with r-ADSCs without fibrin matrix group, positive effects were obtained as compared to other implanted groups by increasing the sciatic functional index significantly (p < 0.05). In addition, the diameter nerve fibers had a significant difference mean in the PLGA conduit coated by laminin and conduit filled with r-ADSCs in fibrin matrix groups relative to the autograft group (p < 0.001). However, G-ratio and amplitude (AMP) results showed that fibrin matrix might have beneficial effects on nerve regeneration but, immunohistochemistry and real-time RT-PCR outcomes indicated that the implanted conduit which filled with r-ADSCs, with or without BDNF-CNPs and AuNPs-CNPs had significantly higher expression of S100 and MBP markers than other conduit implanted groups (p < 0.05). It seems, in this study differential effects of fibrin matrix, could be interfered it with other factors thereby and further studies are required to determine the distinctive effects of fibrin matrix combination with other exogenous factors in peripheral nerve regeneration.

中文翻译：

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大鼠 ADSCs 纤维蛋白基质包裹及 BDNF 和金纳米粒子联合递送对周围神经再生的不同影响

纤维蛋白作为细胞外基质的生物相容性等特性，为细胞的增殖和迁移创造了有利的环境，并且可以作为组织工程中生长因子的储存和释放的储存库。在这项研究中，电纺聚（乳酸-共-乙醇酸）（PLGA）纳米纤维导管的内表面用含有脑源性神经营养因子（BDNF）的层粘连蛋白和壳聚糖纳米颗粒中的金纳米颗粒进行了生物功能化。将大鼠随机分为5组，包括自体移植组作为阳性对照、层粘连蛋白包被并填充DMEM/F12的PLGA导管、层粘连蛋白包被并填充大鼠脂肪干细胞（r-ADSCs）的PLGA导管、PLGA由含有金壳聚糖纳米粒子 (AuNPs-CNPs) 的层粘连蛋白涂层的导管，BDNF-壳聚糖纳米粒子（BDNF-CNPs）填充r-ADSCs或填充r-ADSCs悬浮在纤维蛋白基质中，并将它们植入10 mm大鼠坐骨神经间隙。最终，12 周后评估轴突再生和功能恢复。术后 3 个月，结果显示，PLGA 导管填充 r-ADSCs 无纤维蛋白基质组与其他植入组相比，通过显着增加坐骨神经功能指数获得了积极效果（p < 0.05）。此外，相对于自体移植组，纤维蛋白基质组中层粘连蛋白涂层的 PLGA 导管和填充 r-ADSC 的导管中的直径神经纤维均值有显着差异（p < 0.001）。然而，G 比值和振幅 (AMP) 结果表明，纤维蛋白基质可能对神经再生具有有益作用，但免疫组织化学和实时 RT-PCR 结果表明，植入的导管填充有 r-ADSCs、有或没有 BDNF-CNPs 和AuNPs-CNPs 的 S100 和 MBP 标志物的表达明显高于其他导管植入组 (p < 0.05)。看来，在这项研究中，纤维蛋白基质的不同作用可能会受到其他因素的干扰，因此需要进一步的研究来确定纤维蛋白基质与其他外源性因素在周围神经再生中的独特作用。有或没有 BDNF-CNPs 和 AuNPs-CNPs 的 S100 和 MBP 标志物的表达明显高于其他导管植入组 (p < 0.05)。看来，在这项研究中，纤维蛋白基质的不同作用可能会受到其他因素的干扰，因此需要进一步的研究来确定纤维蛋白基质与其他外源性因素在周围神经再生中的独特作用。有或没有 BDNF-CNPs 和 AuNPs-CNPs 的 S100 和 MBP 标志物的表达明显高于其他导管植入组 (p < 0.05)。看来，在这项研究中，纤维蛋白基质的不同作用可能会受到其他因素的干扰，因此需要进一步的研究来确定纤维蛋白基质与其他外源性因素在周围神经再生中的独特作用。