Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption,ACS Biomaterials Science & Engineering

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Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-08-12 , DOI: 10.1021/acsbiomaterials.1c00735
Ngoc Bao Hyunh ₁ , Cristina Santos Dias Palma ₂ , Rolle Rahikainen ₃ , Ayush Mishra ₁ , Latifeh Azizi ₃ , Enrica Verne ₄ , Sara Ferraris ₄ , Vesa Pekka Hytönen _{3,

5} , Andre Sanches Ribeiro ₂ , Jonathan Massera ₁

Affiliation

The traditional silicate bioactive glasses exhibit poor thermal processability, which inhibits fiber drawing or sintering into scaffolds. The composition of the silicate glasses has been modified to enable hot processing. However, the hot forming ability is generally at the expense of bioactivity. Metaphosphate glasses, on the other hand, possess excellent thermal processability, congruent dissolution, and a tailorable degradation rate. However, due to the layer-by-layer dissolution mechanism, cells do not attach to the material surface. Furthermore, the congruent dissolution leads to a low density of OH groups forming on the glass surface, limiting the adsorption of proteins. It is well regarded that the initial step of protein adsorption is critical as the cells interact with this protein layer, rather than the biomaterial itself. In this paper, we explore the possibility of improving protein adsorption on the surface of phosphate glasses through a variety of surface treatments, such as washing the glass surface in acidic (pH 5), neutral, and basic (pH 9) buffer solutions followed or not by a treatment with (3-aminopropyl)triethoxysilane (APTS). The impact of these surface treatments on the surface chemistry (contact angle, ζ-potential) and glass structure (FTIR) was assessed. In this manuscript, we demonstrate that understanding of the material surface chemistry enables to selectively improve the adsorption of albumin and fibronectin (used as model proteins). Furthermore, in this study, well-known silicate bioactive glasses (i.e., S53P4 and 13-93) were used as controls. While surface treatments clearly improved proteins adsorption on the surface of both silicate and phosphate glasses, it is of interest to note that protein adsorption on phosphate glasses was drastically improved to reach similar protein grafting ability to the silicate bioactive glasses. Overall, this study demonstrates that the limited cell/phosphate glass biological response can easily be overcome through deep understanding and control of the glass surface chemistry.

中文翻译：

用于控制蛋白质吸附的生物可吸收磷酸盐玻璃的表面改性

传统的硅酸盐生物活性玻璃具有较差的热加工性，这会抑制纤维拉伸或烧结成支架。硅酸盐玻璃的成分已经过修改，可以进行热加工。然而，热成型能力通常是以牺牲生物活性为代价的。另一方面，偏磷酸盐玻璃具有出色的热加工性、一致的溶解性和可定制的降解率。然而，由于逐层溶解机制，细胞不会附着在材料表面。此外，全等溶解导致玻璃表面形成低密度的 OH 基团，限制了蛋白质的吸附。众所周知，蛋白质吸附的初始步骤至关重要，因为细胞与该蛋白质层相互作用，而不是生物材料本身。在本文中，我们探索了通过各种表面处理改善磷酸盐玻璃表面蛋白质吸附的可能性，例如在酸性 (pH 5)、中性和碱性 (pH 9) 缓冲溶液中清洗玻璃表面，然后进行处理或不进行处理(3-氨基丙基)三乙氧基硅烷 (APTS)。评估了这些表面处理对表面化学（接触角、ζ 电位）和玻璃结构 (FTIR) 的影响。在这份手稿中，我们证明了对材料表面化学的理解能够选择性地提高白蛋白和纤连蛋白（用作模型蛋白）的吸附。此外，在这项研究中，众所周知的硅酸盐生物活性玻璃（即 S53P4 和 13-93）用作对照。虽然表面处理明显改善了硅酸盐和磷酸盐玻璃表面上的蛋白质吸附，值得注意的是，磷酸盐玻璃上的蛋白质吸附得到显着改善，以达到与硅酸盐生物活性玻璃相似的蛋白质接枝能力。总的来说，这项研究表明，通过深入了解和控制玻璃表面化学，可以轻松克服有限的细胞/磷酸盐玻璃生物反应。

更新日期：2021-09-13

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