ABSTRACT

This study aimed to evaluate the effect of Pulsed Electromagnetic Fields (PEMF) in improving blood flow reduction and tissue necrosis of ischemic animal induced by skin flap. In each experiment, twenty rats (280–320 g) were randomly divided into control group (n = 10) and PEMF (n = 10) group. All of the rats were performed skin flap in back. In the PEMF group, PEMF (1 Hz, 10 mT) was performed in each experiment. In Experiment-1 (n = 20), PEMF was performed for 90 minutes. In Experiment-2 (n = 20), additionally, a blocking film was inserted, and suture was performed to induce necrosis. PEMF was performed for 30 minutes each day for 7 days. As a result of Speckle-Flow Index (SFI) analysis, in the control group, blood flow continued to decrease immediately after the procedure. In the PEMF group, blood flow was remained constant after 30 minutes and increased after 60 minutes. The blood flow in a specific region substantially increased from the initial state. As a result of skin necrosis analysis, the progression rate in the PEMF group was slower than that of the control group. The rate of necrosis in the PEMF group decreased dramatically from the 6th day, and there was a statistically significant difference between the two groups at the 7th day (p < .05). In this study, it was confirmed that PEMF (1 Hz, 10 mT) has a blood flow improvement and skin tissue necrosis alleviation in the ischemic flap animal model.

摘要

本研究旨在评价脉冲电磁场​​（PEMF）对改善皮瓣引起的缺血动物血流减少和组织坏死的作用。每个实验中，20只大鼠（280-320 g）随机分为对照组（n = 10）和PEMF组（n = 10）。所有大鼠均进行背部皮瓣移植。在PEMF组中，每次实验均进行PEMF（1 Hz，10 mT）。在实验 1 (n = 20) 中，PEMF 进行了 90 分钟。在实验2（n = 20）中，另外插入阻挡膜，并进行缝合以诱导坏死。PEMF 每天进行 30 分钟，持续 7 天。斑点流指数（SFI）分析的结果显示，对照组的血流量在手术后立即继续减少。在PEMF组中，血流量在30分钟后保持恒定，并在60分钟后增加。特定区域的血流量较初始状态大幅增加。皮肤坏死分析的结果显示，PEMF 组的进展速度慢于对照组。PEMF 组的坏死率从第 6 天开始急剧下降，第 7 天两组之间存在统计学显着性差异 ( p < .05)。本研究证实PEMF（1 Hz，10 mT）对缺血皮瓣动物模型具有改善血流和减轻皮肤组织坏死的作用。