ABSTRACT

We characterized the bacterial community in shoot and rhizosphere of healthy adult soybean plants grown in two differentially managed soils. Then, these communities were transplanted to seeds to assess their effect on seedling development. The bacterial communities of inoculated soybean seedlings were assessed through 16S rRNA amplicon sequencing and seedlings’ growth was measured. The diversity in the bacterial consortium inoculum prepared from the rhizosphere was higher than those from the shoot. However, the application of bacterial consortium in seedling had no influence on diversity. The resulting bacterial community of seedlings inoculated with bacterial consortium presented an enrichment of specific plant growth-promoting groups, such as Gammaproteobacteria (Enterobacter, Pseudomonas, and Xanthomonas). The predicted potential functions showed an increased abundance of functions related to plant protection and nutrition. The application of bacterial consortium from the shoot of soybean plants grown in the soil with a previous history of soybean cultivation increased the seedling radicle length, hypocotyl length, and total dry weight by 44%, 30%, and 29%, respectively. Our study showed that selecting a consortium of indigenous microbes as inoculants is a potential approach to enhance the use and impacts of plant beneficial microorganisms on plant growth and possibly productivity.

摘要

我们对在两种不同管理的土壤中生长的健康成年大豆植株的枝条和根际细菌群落进行了表征。然后，将这些群落移植到种子中以评估它们对幼苗发育的影响。通过 16S rRNA 扩增子测序评估接种大豆幼苗的细菌群落，并测量幼苗的生长。从根际制备的细菌聚生体接种物的多样性高于地上部。然而，细菌聚生体在幼苗中的应用对多样性没有影响。由此产生的接种细菌群落的幼苗细菌群落呈现出特定植物生长促进群的富集，例如 Gammaproteobacteria（肠杆菌、假单胞菌和黄单胞菌属）。预测的潜在功能显示与植物保护和营养相关的功能更加丰富。在具有大豆栽培历史的土壤中生长的大豆植株芽中的细菌聚生体的应用使幼苗胚根长度、下胚轴长度和总干重分别增加了 44%、30% 和 29%。我们的研究表明，选择一个本地微生物群作为接种剂是一种潜在的方法，可以增强植物有益微生物的使用和对植物生长和可能生产力的影响。