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Potentiation of Antimicrobial Photodynamic Therapy by Curcumin-loaded Graphene Quantum Dots
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2021-08-12 , DOI: 10.1111/php.13503
Sana Mushtaq 1, 2 , Tariq Yasin 3 , Muhammad Saleem 4 , Tianhong Dai 2 , Muhammad Arfat Yameen 1
Affiliation  

Increasing resistance to existing antibiotics by microbes is currently the biggest dilemma. Antimicrobial photodynamic therapy is a promising alternative for the treatment of multidrug-resistant infections. The aim of the current study was to fabricate graphene quantum dots loaded with curcumin as photosensitizer for improved antimicrobial photodynamic therapy. The study involved fabrication of blank and curcumin-loaded graphene quantum dots, their characterizations (TEM, UV-visible and fluorescence emission spectra), cytotoxicity assay, ROS assay and investigation of enhanced antimicrobial photodynamic effect against resistant microbes. The fabrication of blank and loaded graphene quantum dots was confirmed by the observation of peak shift and changes in peak intensity of blank graphene quantum dots, curcumin alone compared with curcumin-loaded graphene quantum dots in UV-visible and fluoresce emission spectra. Cytotoxicity assay showed that 100 µm concentration was not toxic to NIH/3t3 fibroblasts. In ROS assay, the curcumin-loaded formulation showed three-fold increase in ROS production. Blue-light (405 nm) irradiance of 30 J cm−2 and photosensitizer concentration of 100 µm showed ~3.5 log10 enhanced CUF reduction against Pseudomonas aeruginosa, MRSA, Escherichia coli and Candida albicans. In conclusion, curcumin-loaded graphene quantum dots shoed enhanced antimicrobial photodynamic effects and can be used as an alternative effective treatment for resistant infections.

中文翻译:

负载姜黄素的石墨烯量子点增强抗菌光动力疗法

增加微生物对现有抗生素的耐药性是目前最大的困境。抗菌光动力疗法是治疗耐多药感染的一种有前途的替代方法。本研究的目的是制造负载姜黄素的石墨烯量子点作为光敏剂,以改善抗菌光动力疗法。该研究涉及空白和负载姜黄素的石墨烯量子点的制造、它们的表征(TEM、UV-可见和荧光发射光谱)、细胞毒性测定、ROS 测定以及对抗性微生物增强的抗微生物光动力效应的研究。通过观察空白石墨烯量子点的峰位移和峰强度变化,证实了空白和负载石墨烯量子点的制备,在紫外可见和荧光发射光谱中,姜黄素单独与负载姜黄素的石墨烯量子点进行了比较。细胞毒性测定表明,100 µm浓度对 NIH/3t3 成纤维细胞没有毒性。在 ROS 测定中,负载姜黄素的制剂显示 ROS 产生增加了三倍。30 J cm -2的蓝光 (405 nm) 辐照度和 100 µ m的光敏剂浓度显示,针对铜绿假单胞菌、 MRSA 、大肠杆菌白色念珠菌的CUF 降低约 3.5 log 10。总之,负载姜黄素的石墨烯量子点增强了抗菌光动力效应,可用作耐药性感染的替代有效治疗方法。
更新日期:2021-08-12
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