Advantages in biomass pretreatment could be reached by improving lignin removal. In this study, the antioxidant butylated hydroxytoluene (BHT) was evaluated for lignin removal from biomass. In pretreatments performed at 121 °C with 50% (v/v) ethanol as solvent, the presence of BHT resulted in 45.28% and 72.45% lignin and hemicellulose removal, against its absence that resulted in 35.09% and 56.31%, respectively. The use of BHT in lignin removal under these conditions represents an improvement in the pretreatment reducing the biomass recalcitrance. FTIR analyses showed that organosolv pretreatment with BHT influenced band intensity of the pseudo-lignin, while in acid medium similarity was observed among the spectra. The enzymatic hydrolysis of pretreated biomass suffered the influence of hemicellulose and lignin removal. Pretreatments at 121 °C resulted in the highest cellulose conversion with 75% ethanol (58.67%), while pretreated biomass at 160 °C with 0.3% BHT resulted in a 59.04% of glucose yield. The use of BHT increased the lignin solubilization from the biomass in the organosolv pretreatment, increasing the effectiveness of the process, collaboration for further use of lignin.

通过提高木质素去除率可以达到生物质预处理的优势。在这项研究中，评估了抗氧化剂丁基化羟基甲苯 (BHT) 从生物质中去除木质素的能力。在 121 °C 下以 50% (v/v) 乙醇为溶剂进行的预处理中，BHT 的存在导致 45.28% 和 72.45% 的木质素和半纤维素去除，而 BHT 的缺失导致分别为 35.09% 和 56.31%。在这些条件下使用 BHT 去除木质素代表了预处理的改进，减少了生物质的顽固性。FTIR 分析表明，用 BHT 的有机溶剂预处理影响了假木质素的谱带强度，而在酸性介质中观察到光谱之间的相似性。预处理生物质的酶水解受到半纤维素和木质素去除的影响。在 121 °C 下预处理导致最高的纤维素转化率为 75% 乙醇 (58.67%)，而在 160 °C 下使用 0.3% BHT 预处理的生物质导致葡萄糖产量为 59.04%。BHT 的使用增加了有机溶剂预处理中生物质中木质素的溶解，提高了过程的有效性，为进一步使用木质素提供了合作。