Abstract

In this study, we evaluated the in vitro effects of 1–50 μM zearalenone (ZEA), deoxynivalenol (DON) and T-2 toxin (T-2) on rabbit spermatozoa for as much as 8 h of in vitro exposure. Our results indicate that all sperm quality parameters were negatively affected by these fusariotoxins in a time- and dose-dependent manner. The most prominent structure affected by ZEA was the plasma membrane, exhibiting alterations consistent with the onset of apoptosis and reactive oxygen species (ROS) overproduction. This correlated with the most prominent decline of the sperm motility among all selected fusariotoxins. Significant necrotic changes and mitochondrial dysfunction were primarily responsible for the sperm damage in the presence of T-2. Finally, exposure of spermatozoa to DON led to a significant decrease in the DNA integrity. This study may provide new information on the specific mechanisms of action involved in the in vitro toxic behavior of fusariotoxins on male gametes.

摘要

在这项研究中，我们评估了1-50 μM 玉米赤霉烯酮 (ZEA)、脱氧雪腐镰刀菌烯醇 (DON) 和 T-2 毒素 (T-2) 对兔精子长达 8 小时的体外影响。暴露。我们的结果表明，所有精子质量参数都以时间和剂量依赖性方式受到这些镰刀菌毒素的负面影响。受 ZEA 影响的最突出结构是质膜，其表现出与细胞凋亡和活性氧 (ROS) 过量产生一致的变化。这与所有选定的镰刀菌毒素中最显着的精子活力下降有关。显着的坏死变化和线粒体功能障碍是 T-2 存在时精子损伤的主要原因。最后，将精子暴露于 DON 会导致 DNA 完整性显着下降。该研究可能提供有关镰刀菌毒素对雄性配子的体外毒性行为的具体作用机制的新信息。