Background: Cow’s milk allergy (CMA) is the most common IgE-mediated food allergy and Bos d 5 is the major allergen in cow’s milk proteins. More than 60% of the patients with CMA are sensitized to this protein. Methods and Results: A recombinant protein, encoded by a synthetic gene and consisting of reassembled Bos d 5 fragments, was expressed in E. coli strain BL21 (DE3) cells and purified to homogeneity. The B5M lacked relevant IgE-reactivity and allergenic activity compared with Bos d 5 in dot-blot and basophil activation assays. T-cell proliferation experiments demonstrated that B5M preserved the main T cell epitopes of Bos d 5. Immunization of rabbits with B5M induced protective IgG antibodies that blocked the binding of patients’ IgE antibodies to the wild-type allergen and inhibited the degranulation of basophils induced by Bos d 5. Conclusion: Thus, we developed a new strategy, which was based on rational molecular reassembly for allergen-specific immunotherapy (AIT) of CMA and food allergy.
Int Arch Allergy Immunol

中文翻译：

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将 Bos d 5 的过敏原折叠改变为用于牛奶过敏免疫治疗的低过敏性疫苗

背景：牛奶过敏 (CMA) 是最常见的 IgE 介导的食物过敏，而 Bos d 5 是牛奶蛋白中的主要过敏原。超过 60% 的 CMA 患者对这种蛋白质敏感。方法和结果：由合成基因编码并由重新组装的 Bos d 5 片段组成的重组蛋白在大肠杆菌中表达菌株 BL21 (DE3) 细胞并纯化至同质。在斑点印迹和嗜碱性粒细胞活化测定中，与 Bos d 5 相比，B5M 缺乏相关的 IgE 反应性和过敏原活性。T 细胞增殖实验表明，B5M 保留了 Bos d 5 的主要 T 细胞表位。用 B5M 免疫兔子诱导的保护性 IgG 抗体可阻断患者的 IgE 抗体与野生型过敏原的结合，并抑制诱导的嗜碱性粒细胞脱粒。由 Bos d 5.结论：因此，我们开发了一种新策略，该策略基于合理的分子重组，用于 CMA 和食物过敏的过敏原特异性免疫治疗 (AIT)。
Int Arch 过敏免疫