Pullulan is a biocompatible and water-soluble exo-polysaccharide produced by primary strains of the fungus Aureobasidium pullulans. It is frequently used in the pharmaceutical and food industries. In this study, possible cytotoxic effect of pullulan was assessed using the MTT assay in the human breast cancer (MCF-7) cell line. Micronucleus (MN), micronucleus-FISH (MN-FISH), random amplified polymorphic DNA (RAPD-PCR), and comet assays were used to investigate genotoxic and antigenotoxic effects of pullulan against mitomycin C (MMC) (at MN assay) and hydrogen peroxide (at comet assay) in human lymphocytes. Antigenotoxicity was determined using two different applications: 1 h pretreatment and simultaneous treatment. In the MTT assay, pullulan significantly reduced the cell viability at 15.6−2000 μg/mL compared to the control. No significant alterations in MN rates were found in human lymphocytes treated with different concentrations of pullulan compared to the control. In contrast, co-treatment of pullulan and MMC decreased the frequency of MN in almost all the treatment concentrations and durations compared to the MMC. No significant change was observed in the frequency of the centromere-positive C + or negative C- MNi compared to the positive control. In comet assay, pullulan did not affect comet tail intensity compared to the negative control. On the contrary, pullulan in combination with H₂O₂ significantly decreased tail intensity at almost all the concentrations compared to the positive control. The changes occurring in RAPD-PCR profiles following pullulan treatments included an increase or decrease in band intensity and gain or loss of bands. These results indicate that exopolysaccharide Pullulan is not genotoxic; moreover, it possesses a protective effect against MMC and H₂O₂ induced genotoxicity. In breast cancer cells, pullulan induced cytotoxic/anti-proliferative effect.

普鲁兰多糖是一种生物相容性和水溶性的外多糖，由真菌Aureobasidium pullulans 的初级菌株产生. 它经常用于制药和食品行业。在这项研究中，使用 MTT 测定法在人乳腺癌 (MCF-7) 细胞系中评估了普鲁兰多糖可能的细胞毒性作用。微核 (MN)、微核-FISH (MN-FISH)、随机扩增多态性 DNA (RAPD-PCR) 和彗星试验用于研究支链淀粉对丝裂霉素 C (MMC)（在 MN 试验中）和氢的遗传毒性和抗原毒性作用人淋巴细胞中的过氧化物（彗星试验）。使用两种不同的应用来确定抗原毒性：1 小时预处理和同时处理。在 MTT 测定中，与对照相比，支链淀粉在 15.6-2000 μg/mL 时显着降低了细胞活力。与对照相比，用不同浓度的支链淀粉处理的人淋巴细胞中未发现 MN 率的显着变化。相比之下，与 MMC 相比，普鲁兰多糖和 MMC 的共同处理降低了几乎所有处理浓度和持续时间中 MN 的频率。与阳性对照相比，着丝粒阳性 C + 或阴性 C- MNi 的频率没有观察到显着变化。在彗星试验中，与阴性对照相比，普鲁兰多糖不影响彗尾强度。相反，支链淀粉与 H与阳性对照相比，₂ O ₂在几乎所有浓度下都显着降低了尾部强度。普鲁兰多糖处理后 RAPD-PCR 谱中发生的变化包括条带强度的增加或减少以及条带的增加或减少。这些结果表明胞外多糖支链淀粉没有遗传毒性；此外，它对MMC和H ₂ O ₂诱导的遗传毒性具有保护作用。在乳腺癌细胞中，支链淀粉诱导细胞毒性/抗增殖作用。