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Morphological, phytochemical and genetic diversity of threatened Polygonatum verticillatum (L.) All. populations of different altitudes and habitat types in Himalayan region
Physiology and Molecular Biology of Plants ( IF 3.4 ) Pub Date : 2021-08-12 , DOI: 10.1007/s12298-021-01044-9
Renu Suyal 1 , Arun K Jugran 2 , Ranbeer S Rawal 1 , Indra D Bhatt 1
Affiliation  

Polygonatum verticillatum (L.) All. is an important medicinal herb that belongs to the family Asparagaceae. The rhizome of the species is used in Chyavanprash preparation and several other ayurvedic formulations. Numerous active constituents like saponins, alkaloids, phytohormones, flavonoids, antioxidants, lysine, serine, aspartic acid, diosgenin, β-sitosterol, etc. have been reported from this species. In this study, morphological, phytochemical, antioxidant and genetic variations of 11 distant populations of P. verticillatum were measured. Considerably (P < 0.05) higher variations were recorded among different populations of P. verticillatum using morphological, phytochemical and genetic diversity parameters. AGFW (above ground fresh weights); flavonols, FRAP (Ferric ion reducing antioxidant power) and NO (Nitric Oxide scavenging activity) were recorded maximum in Kafni population. Similarly, a significantly higher above and below ground dry weight was recorded in Mayawati and Surmoli populations respectively. Maximum phenolic content, tannins, and DPPH (2,2-diphenyl-1-picrylhydrazyl) activity were recorded in Milam population. A total of 165 individuals from 11 populations were assessed for genetic diversity using inter-simple sequence repeats (ISSR) marker. High genetic diversity (He = 0.35) was recorded in Himkhola and Surmoli populations while it was observed minimum (0.28) in the Mayawati population. Altitude showed a significant positive correlation with tannins (r = 0.674; P < 005) and DPPH (r = 0.820; P < 0.01). Phenol content exhibited a considerably positive relationship with He (r = 0.606; P < 0.05) and BGFW (r = 0.620; P < 0.05), flavonol displayed a positive correlation with Pp% (r = 0.606; P < 0.05). The population structure of P. verticillatum, exhibited that the optimal value of the K was 3 for its populations as determined by the ΔK statistic structure. Among populations, the amount of gene flow is higher (Nm = 1.717) among all sites. Hence, it can be concluded that P. verticillatum populations possess considerable variability in the collected populations. Likewise, the populations from Kafni, Satbunga and Himkhola with higher morphological, phytochemicals and genetic variability were prioritized and therefore recommended for cultivation and mass multiplication to meet the industrial demand for target species.



中文翻译:

受威胁黄精 (L.) All 的形态、植物化学和遗传多样性。喜马拉雅地区不同海拔和栖息地类型的种群

黄精(L.)全部。是天门冬科的重要药材。该物种的根茎用于 Chyavanprash 制剂和其他几种阿育吠陀制剂。从该物种中报道了许多活性成分,如皂苷、生物碱、植物激素、黄酮类化合物、抗氧化剂、赖氨酸、丝氨酸、天冬氨酸、薯蓣皂苷元、β-谷甾醇等。在这项研究中,测量了 11 个遥远的轮枝菌种群的形态、植物化学、抗氧化和遗传变异。显着( P  < 0.05) 不同群体之间的差异较大使用形态学、植物化学和遗传多样性参数。AGFW(地上鲜重);黄酮醇、FRAP(铁离子降低抗氧化能力)和 NO(一氧化氮清除活性)在 Kafni 人群中的记录最高。同样,在 Mayawati 和 Surmoli 种群中分别记录了显着更高的地上和地下干重。在 Milam 种群中记录了最大酚类含量、单宁和 DPPH (2,2-diphenyl-1-picrylhydrazyl) 活性。使用简单序列重复 (ISSR) 标记对来自 11 个种群的 165 个个体进行遗传多样性评估。在 Himkhola 和 Surmoli 种群中记录到高遗传多样性(He = 0.35),而在 Mayawati 种群中观察到最低(0.28)。海拔高度与单宁呈显着正相关(r = 0.674;P  < 005) 和 DPPH (r = 0.820; P  < 0.01)。苯酚含量与 He (r = 0.606; P  < 0.05) 和 BGFW (r = 0.620; P  < 0.05) 呈显着正相关,黄酮醇与 Pp% 呈正相关 (r = 0.606; P  < 0.05)。P. verticillatum的种群结构表明,由 ΔK 统计结构确定,其种群的 K 的最佳值为 3。在人群中,所有位点的基因流量较高(Nm = 1.717)。因此,可以得出结论,P. verticillatum种群在收集的种群中具有相当大的变异性。同样,来自 Kafni、Satbunga 和 Himkhola 的具有较高形态、植物化学物质和遗传变异性的种群被优先考虑,因此建议进行栽培和大规模繁殖,以满足对目标物种的工业需求。

更新日期:2021-08-12
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